NCBP2 / CBP20

nuclear cap binding protein subunit 2, 20kDa

Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2, thereby being required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP2/CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires NCBP1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure.

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Products

Antibodies (16)

Proteins (8)

ELISA Kits (1)

Type

Cell-Based (1)

Over-Expression Lysate (2)

Primary (16)

Recombinant (6)

Target

NCBP2 / CBP20 (25)

Reactivity

Human (17)

Mouse (13)

Rat (3)

Bovine (1)

Monkey (1)

Application

IHC (10)

IHC-P (3)

WB (13)

ELISA (5)

ICC (1)

IF (6)

IP (2)

Peptide-ELISA (2)

Host

rabbit (16)

Isotype

IgG (12)

Clonality

polyclonal pc (16)

Format

96-Well Microplate (1)

Unconjugated (16)

Epitope

aa1-156 (2)

aa106-156 (2)

10-90 aa, N-terminal (1)

Arg21 (1)

N-Terminus (1)

aa1-50 (1)

aa10-59 (1)

Publications

No (25)

Sample Type

Adherent Cell Cultures (1)

Tag

GST (1)

GST, N-terminus (2)

His (1)

Myc-DDK (Flag) (4)

Species

Human (7)

Source

293T Cells (1)

E. coli (2)

HEK 293 Cells (3)

Wheat Germ Extract (2)

Purification

Purified (2)

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NCBP2 / CBP20 Antibody (10-90 aa, N-terminal) LS-C382481

Antibody:

NCBP2 / CBP20 Rabbit anti-Human Polyclonal (10-90 aa, N-terminal) Antibody

Reactivity:

Mouse, Human

Applications:

ELISA, IF, IHC, WB

Format:

Unconjugated

Price:

50 µg/$295; 100 µg/$335; 200 µg/$394

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NCBP2 / CBP20 Antibody (N-Terminus) LS-C368657

Antibody:

NCBP2 / CBP20 Rabbit anti-Human Polyclonal (N-Terminus) Antibody

Reactivity:

Mouse, Bovine, Rat, Human, Monkey

Applications:

ICC, IF, IHC, IHC-P, WB

Format:

Unconjugated

Price:

30 µl/$255; 100 µl/$299; 200 µl/$351

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NCBP2 / CBP20 Antibody (aa1-156) LS-C376198

Antibody:

NCBP2 / CBP20 Rabbit anti-Human Polyclonal (aa1-156) Antibody

Reactivity:

Human

Applications:

ELISA, IHC, IHC-P, WB

Format:

Unconjugated

Price:

50 µl/$294; 100 µl/$360

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NCBP2 / CBP20 Antibody LS-C346336

Antibody:

NCBP2 / CBP20 Rabbit anti-Human Polyclonal Antibody

Reactivity:

Mouse, Rat, Human

Applications:

IF, IHC, WB

Format:

Unconjugated

Price:

20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503

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NCBP2 / CBP20 Antibody LS-C768430

Antibody:

NCBP2 / CBP20 Rabbit anti-Human Polyclonal Antibody

Reactivity:

Mouse, Rat, Human

Applications:

IF, IHC, WB

Format:

Unconjugated

Price:

60 µl/$331; 120 µl/$403; 200 µl/$529

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NCBP2 / CBP20 Antibody (Arg21) LS-C176776

Antibody:

NCBP2 / CBP20 Rabbit anti-Human Polyclonal (Arg21) Antibody

Reactivity:

Mouse, Human

Applications:

IF, IHC, WB

Format:

Unconjugated

Price:

50 µl/$321; 100 µl/$403

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NCBP2 / CBP20 Antibody (aa106-156) LS-C289286

Antibody:

NCBP2 / CBP20 Rabbit anti-Human Polyclonal (aa106-156) Antibody

Reactivity:

Mouse, Human

Applications:

IHC, IP, WB

Format:

Unconjugated

Price:

10 µl/$295; 100 µl/$514

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NCBP2 / CBP20 Antibody (aa1-156) LS-C376199

Antibody:

NCBP2 / CBP20 Rabbit anti-Human Polyclonal (aa1-156) Antibody

Reactivity:

Human

Applications:

ELISA, IHC, IHC-P

Format:

Unconjugated

Price:

50 µl/$294; 100 µl/$360

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NCBP2 / CBP20 Antibody LS-C804514

Antibody:

NCBP2 / CBP20 Rabbit anti-Human Polyclonal Antibody

Reactivity:

Mouse, Human

Applications:

IF, Peptide-ELISA, WB

Format:

Unconjugated

Price:

100 µl/$379; 200 µl/$421

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NCBP2 / CBP20 Antibody (aa1-50) LS-C289284

Antibody:

NCBP2 / CBP20 Rabbit anti-Human Polyclonal (aa1-50) Antibody

Reactivity:

Human

Applications:

IHC, IP

Format:

Unconjugated

Price:

10 µg/$295; 100 µl/$514

Select

NCBP2 / CBP20 Antibody LS-C664458

Antibody:

NCBP2 / CBP20 Rabbit anti-Human Polyclonal Antibody

Reactivity:

Human

Applications:

ELISA, WB

Format:

Unconjugated

Price:

100 µg/$341

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NCBP2 / CBP20 Antibody LS-C822783

Antibody:

NCBP2 / CBP20 Rabbit anti-Human Polyclonal Antibody

Reactivity:

Mouse, Human

Applications:

ELISA, WB

Format:

Unconjugated

Price:

100 µg/$357

Select

NCBP2 / CBP20 Antibody (aa10-59) LS-C118927

Antibody:

NCBP2 / CBP20 Rabbit anti-Human Polyclonal (aa10-59) Antibody

Reactivity:

Mouse, Human

Applications:

Peptide-ELISA, WB

Format:

Unconjugated

Price:

50 µl/$334; 100 µl/$397

Select

NCBP2 / CBP20 Antibody (aa106-156) LS-C286912

Antibody:

NCBP2 / CBP20 Rabbit anti-Human Polyclonal (aa106-156) Antibody

Reactivity:

Mouse, Human

Applications:

IHC

Format:

Unconjugated

Price:

1 µg/$295; 100 µl/$532

Select

NCBP2 / CBP20 Antibody LS-C658950

Antibody:

NCBP2 / CBP20 Rabbit anti-Human Polyclonal Antibody

Reactivity:

Mouse, Human

Applications:

WB

Format:

Unconjugated

Price:

100 µl/$391

Select

NCBP2 / CBP20 Antibody LS-C394126

Antibody:

NCBP2 / CBP20 Rabbit anti-Human Polyclonal Antibody

Reactivity:

Mouse, Human

Applications:

WB

Format:

Unconjugated

Price:

100 µl/$386

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Human NCBP2 / CBP20 Protein (Recombinant GST, N-terminus) (aa1-99) - LS-G61871

Source:

Wheat Germ Extract

Tag:

GST, N-terminus

Price:

10 µg/$479; 25 µg/$670

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Human NCBP2 / CBP20 Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G64722

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

17.8 kDa

Price:

20 µg/$1,107

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Human NCBP2 / CBP20 Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G64723

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

11.8 kDa

Price:

20 µg/$1,107

Select

Human NCBP2 / CBP20 Protein (Recombinant GST, N-terminus) (aa1-156) - LS-G56417

Source:

E. coli

Tag:

GST, N-terminus

Price:

1 mg/$1,355; 100 µg/$420; 20 µg/$323

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Human NCBP2 / CBP20 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G74013

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

17.8 kDa

Price:

20 µg/$150

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Human NCBP2 / CBP20 Protein (Recombinant GST) (Full Length) - LS-G32674

Source:

Wheat Germ Extract

Tag:

GST

Molecular Weight:

44.4 kDa

Price:

10 µg/$479; 25 µg/$670

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Mouse/Human NCBP2 / CBP20 (Cell-Based ELISA) ELISA Kit - LS-F3120

Type:

Cell-Based

Format:

96-Well Microplate

Reactivity:

Mouse, Human

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Adherent Cell Cultures

Price:

1 Plate/$618

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Human NCBP2 / CBP20 Protein (Recombinant His) - LS-G966

Source:

E. coli

Tag:

His

Price:

100 µg/$361; 500 µg/$739; 20 µg/$261

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NCBP2 / CBP20 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G101018

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

17.8 kDa

Price:

100 µg/$494

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