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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NAMPT / Visfatin Antibody (aa400‑450) LS‑C285931
Visfatin antibody LS-C285931 is an unconjugated rabbit polyclonal antibody to Visfatin (NAMPT) from human, mouse, rat and other species. Validated for IF and IHC.
Catalog
Size
Price
LS-C285931-100
100 µl
$415
Description
Visfatin antibody LS-C285931 is an unconjugated rabbit polyclonal antibody to Visfatin (NAMPT) from human, mouse, rat and other species. Validated for IF and IHC.
Target
Human NAMPT / Visfatin
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:500)
  • Immunofluorescence (1:50 - 1:500)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Epitope
aa400-450
Specificity
Region between residue 400 and 450 of human pre-B-cell colony enhancing factor using the numbering given in SwissProt entry P43490 (GeneID 10135).
Usage
Immunohistochemistry: Antigen retrieval is recommended. Antigen retrieval with citrate buffer will enhance staining. Likely to work with frozen sections. In some cases, the antibody may be diluted further than indicated. Human controls: Placenta.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
TBS, 0.09% Sodium Azide, 0.1% BSA
Storage
Store at 2°C to 8°C for up to 1 year.
Restrictions
For research use only.
About NAMPT / Visfatin
P43490 NM_005746 NP_005737.1

Popular NAMPT / Visfatin Products

Western blot of NAMPT / Visfatin antibody.
Species: Mouse, Human
Applications: Western blot
Cell lysates of 293T (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Species: Human
Applications: Western blot, ELISA
Western blot of NAMPT / Visfatin antibody.
Species: Rat
Applications: Western blot
NAMPT antibody ARP42255_T100-NP_005737-PBEF1(pre-B-cell colony enhancing factor 1) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Guinea pig, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, Western blot
Western blot of NAMPT in human tumor cell lines at 1:1000. Lanes: A. HeLa, B. Jurkat, C. Daudi, D. HEK 293, E. Rh30, F. A375, G. T98G, H. HCT-116, I. Hep-2.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Chicken, Xenopus, Zebrafish
Applications: Western blot, ELISA

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Images

Immunohistochemistry

Detection of Human PBEF by Immunohistochemistry. Sample: FFPE section of human fetal membrane. Antibody: Affinity purified rabbit anti-PBEF used at a dilution of 1:500. Detection: DAB.
Detection of Human PBEF by Immunohistochemistry. Sample: FFPE section of human fetal membrane. Antibody: Affinity purified rabbit anti-PBEF used at a dilution of 1:500. Detection: DAB.

Immunohistochemistry

Detection of Human PBEF by Immunohistochemistry. Sample: FFPE section of human fetal membrane. Antibody: Affinity purified rabbit anti-PBEF used at a dilution of 1:500. Detection: DAB.
Detection of Human PBEF by Immunohistochemistry. Sample: FFPE section of human fetal membrane. Antibody: Affinity purified rabbit anti-PBEF used at a dilution of 1:500. Detection: DAB.

Immunohistochemistry

Detection of Human PBEF by Immunohistochemistry. Sample: FFPE section of human fetal membrane. Antibody: Affinity purified rabbit anti-PBEF used at a dilution of 1:500. Detection: DAB.
Detection of Human PBEF by Immunohistochemistry. Sample: FFPE section of human fetal membrane. Antibody: Affinity purified rabbit anti-PBEF used at a dilution of 1:500. Detection: DAB.

Immunohistochemistry

Detection of Human PBEF by Immunohistochemistry. Sample: FFPE section of human fetal membrane. Antibody: Affinity purified rabbit anti-PBEF used at a dilution of 1:500. Detection: DAB.
Detection of Human PBEF by Immunohistochemistry. Sample: FFPE section of human fetal membrane. Antibody: Affinity purified rabbit anti-PBEF used at a dilution of 1:500. Detection: DAB.

Requested From: United States
Date Requested: 12/17/2018
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