Visfatin antibody LS-C9513 is an unconjugated rabbit polyclonal antibody to Visfatin (NAMPT) from human and chicken. Validated for ELISA and WB.
(tested or 100% immunogen sequence identity)
Synthetic peptide corresponding to aa 40-56 (R40EKKTENSKLRKVKYEE56) of human visfatin. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Chicken (100%); Marmoset, Mouse, Rat, Hamster, Bovine, Horse, Pig, Opossum, Platypus (94%); Panda, Dog, Xenopus (88%).
Recognizes human visfatin. Weakly cross-reacts with mouse visfatin.
Suitable for use in ELISA and Western Blot. ELISA (Direct and Indirect): 1:2000-1:5000. Western Blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 with 5% skim milk. Incubation time: 1 hour at RT.
PBS, pH 7.4. Contains no preservatives.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.