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Catalog Number Size Price
LS-C744771-25 25 µl (1 mg/ml) $304 
N-Acylmannosamine-1-Dehydrogenase Antibody - Western Blot of Goat anti-N-Acylmannoseamide 1-Dehydrogenase Antibody Biotin Conjugated. Lane 1: N-Acylmannoseamide 1-Dehydrogenase. Load: 50 ng per lane. Primary antibody: Goat anti-N-Acylmannoseamide 1-Dehydrogenase Antibody Biotin Conjugated 1:1,000 overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 27.5 kDa, observed at 30 kDa for N-Acylmannoseamide 1-Dehydrogenase.
N-Acylmannosamine-1-Dehydrogenase Antibody - Goat anti N-acylmanosamino-1-Dehydrogenase antibody was used to detect purified N-acylmanosamino-1-Dehydrogenase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated Donkey anti goat. Images were collected using the BioRad VersaDoc System.
N-Acylmannosamine-1-Dehydrogenase Antibody - Western Blot of Goat anti-N-Acylmannoseamide 1-Dehydrogenase Antibody Biotin Conjugated. Lane 1: N-Acylmannoseamide 1-Dehydrogenase. Load: 50 ng per lane. Primary antibody: Goat anti-N-Acylmannoseamide 1-Dehydrogenase Antibody Biotin Conjugated 1:1,000 overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 27.5 kDa, observed at 30 kDa for N-Acylmannoseamide 1-Dehydrogenase.
N-Acylmannosamine-1-Dehydrogenase Antibody - Goat anti N-acylmanosamino-1-Dehydrogenase antibody was used to detect purified N-acylmanosamino-1-Dehydrogenase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated Donkey anti goat. Images were collected using the BioRad VersaDoc System.
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Polyclonal Goat anti‑Pseudomonas N‑Acylmannosamine‑1‑Dehydrogenase Antibody (Liquid, Biotin, WB) LS‑C744771

Polyclonal Goat anti‑Pseudomonas N‑Acylmannosamine‑1‑Dehydrogenase Antibody (Liquid, Biotin, WB) LS‑C744771

Antibody:
N-Acylmannosamine-1-Dehydrogenase Goat anti-Pseudomonas Polyclonal (Liquid) (Biotin) Antibody
Application:
WB, IP, ELISA
Reactivity:
Pseudomonas, Bacteria
Format:
Biotin, Liquid
Price
Catalog Number
$304
LS-C744771-25
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
N-Acylmannosamine-1-Dehydrogenase Goat anti-Pseudomonas Polyclonal (Liquid) (Biotin) Antibody
Application:
WB, IP, ELISA
Reactivity:
Pseudomonas, Bacteria
Format:
Biotin, Liquid

Specifications

Description
N-Acylmannosamine-1-Dehydrogenase antibody LS-C744771 is a biotin-conjugated goat polyclonal antibody to N-Acylmannosamine-1-Dehydrogenase from pseudomonas. It is reactive with bacteria and pseudomonas. Validated for ELISA, IP and WB.
Target
Pseudomonas N-Acylmannosamine-1-Dehydrogenase
Host
Goat
Reactivity
Pseudomonas, Bacteria (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Liquid
Immunogen
N-Acyl Mannosamine-1-Dehydrogenase [Recombinant Sequence from Pseudomonas expressed in E.coli ]
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified N-Acylmannoseamine-1-Dehydrogenase [Recombinant from Pseudomonas]. Cross reactivity against N-Acylmannoseamine-1-Dehydrogenase from other sources is unknown.
Applications
  • Western blot (1:250 - 1:1000)
  • Immunoprecipitation (1:100)
  • ELISA (1:1000 - 1:5000)
Usage
Applications should be user optimized.
Presentation
10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 4/18/2024