Human MYO5A / Myosin V

Goat

Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence C-ETKQLELDLN, from the internal region of the protein sequence according to NP_000250.3NP_001135967.1.

aa1063-1072

Human MYO5A. This antibody is expected to recognize both reported isoforms (NP_000250.3; NP_001135967.1).

• Immunofluorescence (1 - 3 µg/ml)
• Western blot (1 - 3 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:8000)

Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 200kD band observed in lysates of cell line Jurkat (calculated MW of 215kD according to NP_000250.3). Recommended concentration: 1-3 ug/ml. Immunofluorescence: Transverse sections of Mouse Skeletal Muscle shows staining of the fiber membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodies (Rder et al, Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):2031-6; PMID: 20133847). Recommended concentration: 1-3 ug/ml.

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9Y4I1 NCBI: NM_000259 NP_000250.3