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Catalog Number Size Price
LS-C682081-50 50 µg $271 
LS-C682081-100 100 µg $311 
Myc Tag Antibody - Immunofluorescence staining of transfected HEK293 cells diluted at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L).
Myc Tag Antibody - WB: Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6ug/ml Lane 1:Recombinant Myc-tagged fusion Protein at 50ng Lane 2:Recombinant Myc-tagged fusion Protein at 25ng Lane 3:Recombinant Myc-tagged fusion Protein at 12.5ng Lane 4:Recombinant Myc-tagged fusion Protein at 6.25ng Lane 5:Recombinant Myc-tagged fusion Protein at 3.125ng Lane 6:Recombinant Myc-tagged fusion Protein at 1.5625ng Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 50kd Observed band size: 50kd
Myc Tag Antibody - Immunoprecipitating MYC-tag with transfected HEK293  Lane 1: Mouse control IgG (1µg) instead of product in transfected HEK293 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the Secondary antibody (1/2000) Lane 2: Product (6µg) + transfected HEK293 whole cell lysate (1mg)  Lane 3: Transfected HEK293 whole cell lysate (10µg)
Myc Tag Antibody - Immunofluorescence staining of transfected HEK293 cells diluted at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L).
Myc Tag Antibody - WB: Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6ug/ml Lane 1:Recombinant Myc-tagged fusion Protein at 50ng Lane 2:Recombinant Myc-tagged fusion Protein at 25ng Lane 3:Recombinant Myc-tagged fusion Protein at 12.5ng Lane 4:Recombinant Myc-tagged fusion Protein at 6.25ng Lane 5:Recombinant Myc-tagged fusion Protein at 3.125ng Lane 6:Recombinant Myc-tagged fusion Protein at 1.5625ng Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 50kd Observed band size: 50kd
Myc Tag Antibody - Immunoprecipitating MYC-tag with transfected HEK293  Lane 1: Mouse control IgG (1µg) instead of product in transfected HEK293 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the Secondary antibody (1/2000) Lane 2: Product (6µg) + transfected HEK293 whole cell lysate (1mg)  Lane 3: Transfected HEK293 whole cell lysate (10µg)
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Monoclonal Mouse Myc Tag Antibody (clone 5F9F9, IF, WB) LS‑C682081

Monoclonal Mouse Myc Tag Antibody (clone 5F9F9, IF, WB) LS‑C682081

Antibody:
Myc Tag Mouse Monoclonal (5F9F9) Antibody
Application:
IF, WB, IP, ELISA
Format:
Unconjugated, Unmodified
Price
Catalog Number
$271
LS-C682081-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Myc Tag Mouse Monoclonal (5F9F9) Antibody
Application:
IF, WB, IP, ELISA
Format:
Unconjugated, Unmodified

Specifications

Description
Myc Tag antibody LS-C682081 is an unconjugated mouse monoclonal antibody to Myc Tag. Validated for ELISA, IF, IP and WB.
Host
Mouse
Clonality
IgG1 Monoclonal
Clone
5F9F9
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
EQKLISEEDL (Myc) synthetic peptide conjugate to KLH
Applications
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • ELISA
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/25/2024