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Myc Tag Antibody (aa410‑419, HRP) LS‑C154273

Catalog Number Size Price
LS-C154273-100 100 µg (1 mg/ml) $425 
Antibody

Myc Tag Antibody (aa410‑419, HRP) LS‑C154273

Antibody:
Rabbit Polyclonal to Myc Tag
Application:
IHC, WB, ELISA
Format:
Horseradish Peroxidase, Unmodified
Price
Catalog Number
$425 
LS-C154273-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Myc Tag
Application:
IHC, WB, ELISA
Format:
Horseradish Peroxidase, Unmodified

Specifications

Description
Myc Tag antibody LS-C154273 is an HRP-conjugated rabbit polyclonal antibody to Myc Tag. Validated for ELISA, IHC and WB.
Host
Rabbit
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC (1:500 - 1:2000)
  • Western blot (1:2000 - 1:5000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.
Epitope
aa410-419
Specificity
This affinity purified antibody is directed against human c-myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-Rabbit Serum.
Usage
Anti-myc has utility to detect the fusion protein of the myc epitope cloned along with the target gene. As such, anti-myc/myc can be used to identify fusion proteins containing the myc epitope. The antibody recognizes the Myc tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and myc containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA, 0.01% Gentamicin sulfate
Reconstitution
Reconstitute in deionized water
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 8/22/2019
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