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Catalog Number Size Price
LS-C153853-100 100 µg (0.91 mg/ml) $425 
MYB / c-Myb Antibody - Western blot of Rabbit c-Myb pT486 antibody. Lane 1: Cos7 cells were either null for c-Myb. Lane 2: Cos7 cell lysates transfected with c-myb, no treatment. Lane 3: Cos7 cell lysates transfected with c-Myb and treated with okadaic acid to include phosphorylation of c-Myb. Load: 35 ug per lane. Primary antibody: The left blot was probed with anti-c-Myb antibody, the right blot was probed with anti-c-Myb pT486 antibody at 1:2500 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: PBS-Tween (0.05%) overnight at 4C. Predicted/Observed size: 75, 98, and 125kDa corresponding to overexpressed c-Myb (arrow): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively.

Polyclonal Rabbit anti‑Mouse MYB / c‑Myb Antibody (phospho‑Thr486, WB) LS‑C153853

Polyclonal Rabbit anti‑Mouse MYB / c‑Myb Antibody (phospho‑Thr486, WB) LS‑C153853

Antibody:
Rabbit Polyclonal to Mouse MYB / c-Myb
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C153853-100
100 µg
0.91 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Mouse MYB / c-Myb
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
C-Myb antibody LS-C153853 is an unconjugated rabbit polyclonal antibody to mouse c-Myb (MYB) (pThr486). Validated for ELISA and WB.
Target
Mouse MYB / c-Myb
Synonyms
MYB | C-myb_CDS | C-myb8B_CDS | C-myb protein (140 AA) | C-myb10A_CDS | C-myb13A_CDS | Efg | Transcriptional activator Myb | C-myb | C-myb14A_CDS | Cmyb | Proto-oncogene c-Myb
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Predicted
Human, Chimpanzee, Gorilla, Orangutan, Rat, Horse, Opossum, Pig, Platypus, Spider monkey (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot (1:2500)
  • ELISA (1:5000 - 1:16000)
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse c-Myb protein surrounding amino acid residue 486.
Epitope
pThr486
Specificity
Reactivity occurs against mouse c-Myb pT486 protein and the antibody is specific for the phosphorylated form of the protein. This antibody reacts with endogenous c-Myb protein and also SUMOylated overexpressed c-Myb protein in transfected Cos7 cells. Reactivity with non-phosphorylated mouse c-Myb is minimal by ELISA and western blot. A BLAST analysis was used to suggest reactivity with c-Myb from mouse based on a 100% homology with the immunizing sequence. Expect reactivity with c-Myb from rat, pig, human, horse, platypus, opossum, orangutan, spider monkey, bonobo, gorilla, and chimpanzee; with a v-myb myeloblastosis viral oncogene homolog, all isoforms (also avian version) from human; and with a protooncogene c-myb, and a v-myb myeloblastosis viral oncogene homolog (avian), both from cattle, all based on a 93% homology with the immunizing sequence. Cross-reactivity with c-Myb from other sources has not been determined.
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting using M1 cells which express endogenous wild-type c-Myb protein and in COS7 cells transfected with c-Myb. Specific conditions for reactivity and detection of phosphorylated c-Myb should be optimized by the end user. Expect a band approximately ~75 kD in size corresponding to phosphorylated c-Myb by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MYB / c-Myb
P10242 NM_005375 NP_005366.2

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Requested From: United States
Date Requested: 6/5/2020