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Antibodies > MVP / VAULT1 > LS-C662484
 
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Catalog Number Size Price
LS-C662484-10 10 µg $318 
LS-C662484-100 100 µg $470 
MVP / VAULT1 Antibody - Immunohistochemistry - Anti-MVP Picoband antibody
MVP / VAULT1 Antibody - Immunohistochemistry - Anti-MVP Picoband antibody
MVP / VAULT1 Antibody - Immunohistochemistry - Anti-MVP Picoband antibody
MVP / VAULT1 Antibody - Immunohistochemistry - Anti-MVP Picoband antibody
MVP / VAULT1 Antibody - IF analysis of MVP using anti-MVP antibody MVP was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-MVP Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MVP / VAULT1 Antibody - Western blot analysis of MVP using anti-MVP antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates,Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PANC-1 whole cell lysates, Lane 5: human SGC-7901 whole cell lysates, Lane 6: human MDA-MB-231 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MVP antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MVP at approximately 99KD. The expected band size for MVP is at 99KD.
MVP / VAULT1 Antibody - Western blot - Anti-MVP Picoband antibody
MVP / VAULT1 Antibody - Western blot - Anti-MVP Picoband antibody
MVP / VAULT1 Antibody - Flow Cytometry analysis of U-87 cells using anti-MVP antibody. Overlay histogram showing U-87 cells stained with anti-MVP antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MVP Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
MVP / VAULT1 Antibody - Flow Cytometry analysis of Hela cells using anti-MVP antibody. Overlay histogram showing Hela cells stained with anti-MVP antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MVP Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
MVP / VAULT1 Antibody - Immunohistochemistry - Anti-MVP Picoband antibody
MVP / VAULT1 Antibody - Immunohistochemistry - Anti-MVP Picoband antibody
MVP / VAULT1 Antibody - Immunohistochemistry - Anti-MVP Picoband antibody
MVP / VAULT1 Antibody - Immunohistochemistry - Anti-MVP Picoband antibody
MVP / VAULT1 Antibody - IF analysis of MVP using anti-MVP antibody MVP was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-MVP Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MVP / VAULT1 Antibody - Western blot analysis of MVP using anti-MVP antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates,Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PANC-1 whole cell lysates, Lane 5: human SGC-7901 whole cell lysates, Lane 6: human MDA-MB-231 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MVP antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MVP at approximately 99KD. The expected band size for MVP is at 99KD.
MVP / VAULT1 Antibody - Western blot - Anti-MVP Picoband antibody
MVP / VAULT1 Antibody - Western blot - Anti-MVP Picoband antibody
MVP / VAULT1 Antibody - Flow Cytometry analysis of U-87 cells using anti-MVP antibody. Overlay histogram showing U-87 cells stained with anti-MVP antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MVP Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
MVP / VAULT1 Antibody - Flow Cytometry analysis of Hela cells using anti-MVP antibody. Overlay histogram showing Hela cells stained with anti-MVP antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MVP Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human MVP / VAULT1 Antibody (IHC, WB) LS‑C662484

Polyclonal Rabbit anti‑Human MVP / VAULT1 Antibody (IHC, WB) LS‑C662484

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Antibody:
MVP / VAULT1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662484-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
MVP / VAULT1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
VAULT1 antibody LS-C662484 is an unconjugated rabbit polyclonal antibody to human VAULT1 (MVP). Validated for ELISA, Flow, ICC, IHC and WB.
Target
Human MVP / VAULT1
Synonyms
MVP | Major vault protein | VAULT1 | LRP
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
E. coli-derived human MVP recombinant protein (Position: A2-H259).
Specificity
Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Western blot
  • Flow Cytometry
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MVP / VAULT1
Q14764 NM_017458 NP_059447.2

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Featured Products

MVP / VAULT1 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
PathPlus™ MVP / VAULT1 Antibody (clone 2H3‑1A6) LS‑B6201
Species: Human
Applications: IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Product
MVP / VAULT1 Antibody (clone 1011, Concentrated) LS‑C202625
Species: Mouse
Applications: IHC, IHC - Frozen, Western blot
Product
MVP / VAULT1 Antibody (clone MVP‑37, Concentrated) LS‑C202620
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
MVP / VAULT1 ELISA Kit
Human MVP / VAULT1 (Sandwich ELISA) ELISA Kit - LS-F9931
Reactivity: Human
Range: 0.156-10 ng/ml
MVP / VAULT1 ELISA Kit
Mouse MVP / VAULT1 (Sandwich ELISA) ELISA Kit - LS-F15890
Reactivity: Mouse
Range: 0.156-10 ng/ml
Product
Mouse GAD65 Protein (Recombinant) (Met1-Leu585) - LS-G98163
Source: Baculovirus
Tag: None
See all MVP / VAULT1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
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