Fungus Mushroom Tyrosinase
(tested or 100% immunogen sequence identity)
ELISA: In a simple ELISA with 1 ug of antigen coated per well, the 50% OD was observed at 1/20000. Under the same conditions, the end point was observed at >1/400000. Alkaline phosphatase conjugated anti-sheep was used for detection. All incubations at 18°C using pNPP substrate, with OD measurements at 405 nm.
Serum, 0.09% Sodium Azide