Human Muscle Actin

Mouse

Human, Rat, Rabbit (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

HHF35 + MSA/953

Unconjugated

Protein G purified

Unmodified. Also available Azide-free, BSA-free, Ready-to-Use.

Recombinant human actin fragment.

Human Actin, Muscle

• IHC - Paraffin (0.25 - 0.5 µg/ml)

This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas). Optimal dilution of the pan Muscle Actin antibody cocktail should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.