(tested or 100% immunogen sequence identity)
- IHC - Paraffin (10 µg/ml)
- Western blot
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Un-glycosylated, serine- and threonine-free synthetic peptide from the N-terminus of human MUC5B
Reacts with Mucin 5B (MUC5B). Does not react with human Mucin 5A or 5C
Western blot: Bands in the high molecular weight range of 200kDa or above are expected for highly glycosylated proteins. Immunohistchemistry: Paraffin sections. Deglycosylating the tissue is critical to expose epitope. There are a number of deglycosylation protocols available and a discussion of these related to Mucin can be found in Cao, Y, et al (1997) J Histochem Cytochem, 45:1547-57 (1997). ELISA: Optimal working dilutions must be determined by end user.
PBS, pH 7.6, 0.25 M NaCl, 0.1% Sodium Azide
Store at 2°C to 8°C for 6 months.
For research use only.
This antibody carries the LSBio 100% Guarantee.