Mesothelin antibody LS-B10593 is an unconjugated mouse monoclonal antibody to human Mesothelin (MSLN). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Human MSLN / Mesothelin
(tested or 100% immunogen sequence identity)
Protein A purified
- IHC - Paraffin (15 µg/ml)
- Western blot (1:1000)
- ELISA (1:10000 - 1:50000)
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This antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to the extracellular domain of human mesothelin.
Cross reactivity with homologues from other sources has not been tested.
This antibody has been tested for use in immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kD in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrieval is recommended. Block tissues with 1% BSA in PBS for 30 min at 23° C. Antibodies are diluted in 1% BSA and reacted with tissue for 60 min at room temperature.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.