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Catalog Number	Size	Price
LS-C357477-10	10 µg	$318
LS-C357477-100	100 µg (0.5 mg/ml)	$470

MSLN / Mesothelin Antibody - Mesothelin antibody IHC-paraffin: Human Lung Cancer Tissue.

MSLN / Mesothelin Antibody - Western blot analysis of Mesothelin using anti-Mesothelin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mesothelin antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Mesothelin at approximately 69KD. The expected band size for Mesothelin is at 69KD.

MSLN / Mesothelin Antibody - Mesothelin antibody Western blot. All lanes: Anti Mesothelin at 0.5 ug/ml. WB: Recombinant Human Mesothelin Protein 0.5ng. Predicted band size: 43 kD. Observed band size: 43 kD.

MSLN / Mesothelin Antibody - Flow Cytometry analysis of SiHa cells using anti-Mesothelin antibody. Overlay histogram showing SiHa cells stained with anti-Mesothelin antibody (Blue line). Mesothelin cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Mesothelin Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Polyclonal Rabbit anti‑Human MSLN / Mesothelin Antibody (aa306‑576, IHC, WB) LS‑C357477

Name: Anti-MSLN / Mesothelin Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C357477-10
Price: 318.0000 USD
Availability: InStock

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Note: This antibody replaces LS-C388858

Antibody:

MSLN / Mesothelin Rabbit anti-Human Polyclonal (aa306-576) Antibody

Application:

IHC, IHC-P, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C357477-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

MSLN / Mesothelin Rabbit anti-Human Polyclonal (aa306-576) Antibody

Application:

IHC, IHC-P, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

Mesothelin antibody LS-C357477 is an unconjugated rabbit polyclonal antibody to human Mesothelin (MSLN) (aa306-576). Validated for ELISA, IHC and WB.

Target

Human MSLN / Mesothelin

Synonyms

See All MSLN / Mesothelin Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E.coli-derived human Mesothelin recombinant protein (Position: K306-L576). Human Mesothelin shares 57% and 56% amino acid (aa) sequence identity with mouse and rat Mesothelin, respectively.

Epitope

aa306-576

Specificity

Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). .

Applications

IHC
IHC - Paraffin
Western blot
ELISA

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About MSLN / Mesothelin

Uniprot: Q13421 NCBI: NM_013404 NP_037536.2