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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MRPS27 Antibody (clone 3B9) LS‑C175180
MRPS27 antibody LS-C175180 is an unconjugated mouse monoclonal antibody to human MRPS27. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C175180-100
100 µl (1 mg/ml)
$335
Description
MRPS27 antibody LS-C175180 is an unconjugated mouse monoclonal antibody to human MRPS27. Validated for Flow, IHC and WB.
Target
Human MRPS27
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [3B9]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MRPS27 antibody was raised against human recombinant protein fragment corresponding to amino acids 151-414 of human MRPS27 (NP_055899) produced in E. coli.
Specificity
Human MRPS27
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About MRPS27
Q92552 D87453 Q92552

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Images

Immunohistochemistry

IHC of paraffin-embedded Human colon tissue using anti-MRPS27 mouse monoclonal antibody.
IHC of paraffin-embedded Human colon tissue using anti-MRPS27 mouse monoclonal antibody.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using g anti-MRPS27 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot of extracts (35 ug) from 9 different cell lines by using g anti-MRPS27 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MRPS27 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MRPS27 antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Human colon tissue using anti-MRPS27 mouse monoclonal antibody.
IHC of paraffin-embedded Human colon tissue using anti-MRPS27 mouse monoclonal antibody.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using g anti-MRPS27 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot of extracts (35 ug) from 9 different cell lines by using g anti-MRPS27 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MRPS27 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MRPS27 antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Human colon tissue using anti-MRPS27 mouse monoclonal antibody.
IHC of paraffin-embedded Human colon tissue using anti-MRPS27 mouse monoclonal antibody.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using g anti-MRPS27 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot of extracts (35 ug) from 9 different cell lines by using g anti-MRPS27 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MRPS27 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MRPS27 antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Human colon tissue using anti-MRPS27 mouse monoclonal antibody.
IHC of paraffin-embedded Human colon tissue using anti-MRPS27 mouse monoclonal antibody.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using g anti-MRPS27 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot of extracts (35 ug) from 9 different cell lines by using g anti-MRPS27 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MRPS27 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MRPS27 antibody, and then analyzed by flow cytometry.

Requested From: United States
Date Requested: 11/17/2018
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