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Catalog Number Size Price
LS-C18848-100 100 µg (1.17 mg/ml) $498 
MRE11A / MRE11 Antibody - Anti-Mre11 (S. cerevisiae) Antibody - Chromatin Immunoprecipitation (ChIP). Chromatin Immunoprecipitation (ChIP) using Affinity Purified Mre11 (S. cerevisiae) antibody. A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes). After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin. Chromatin was immunoprecipitated with the antibody at the stated dilutions. immune complexes were captured using polyacrylamide bead linked secondary antibodies. The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus). PCR products were displayed on a polyacrylamide gel, stained with SyBR Green (Invitrogen), and detected using a Fuji scanning fluorimeter. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.

Polyclonal Rabbit anti‑Saccharomyces cerevisiae MRE11A / MRE11 Antibody (WB) LS‑C18848

Polyclonal Rabbit anti‑Saccharomyces cerevisiae MRE11A / MRE11 Antibody (WB) LS‑C18848

Antibody:
MRE11A / MRE11 Rabbit anti-Saccharomyces cerevisiae Polyclonal Antibody
Application:
WB, ELISA, ChrIP
Reactivity:
Saccharomyces cerevisiae
Format:
Unconjugated, Unmodified
Price
Catalog Number
$498
LS-C18848-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MRE11A / MRE11 Rabbit anti-Saccharomyces cerevisiae Polyclonal Antibody
Application:
WB, ELISA, ChrIP
Reactivity:
Saccharomyces cerevisiae
Format:
Unconjugated, Unmodified

Specifications

Description
MRE11 antibody LS-C18848 is an unconjugated rabbit polyclonal antibody to saccharomyces cerevisiae MRE11 (MRE11A). Validated for ChrIP, ELISA and WB.
Target
Saccharomyces cerevisiae MRE11A / MRE11
Synonyms
MRE11A | AT-like disease | Endo/exonuclease Mre11 | HNGS1 | MRE11 | MRE11 homolog 1 | MRE11 homolog A | MRE11B | ATLD
Host
Rabbit
Reactivity
Saccharomyces cerevisiae (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 575-600 of Saccharomyces cerevisiae (baker's yeast) Mre11 protein.
Specificity
A BLAST analysis was used to suggest that little cross reactivity should be expected with this protein from other sources.
Applications
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000 - 1:50000)
  • Chromatin Immunoprecipitation
Usage
This affinity purified antibody has been tested for use in ELISA and by ChIP assay. Specific conditions for reactivity should be optimized by the end user.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MRE11A / MRE11
P49959 NM_005591 NP_005582.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022