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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MPO / Myeloperoxidase Antibody IHC‑plus™ LS‑B6695
Note: This antibody replaces LS-C93836
Myeloperoxidase antibody LS-B6695 is an unconjugated rabbit polyclonal antibody to human Myeloperoxidase (MPO). Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6695-500
500 µg (1 mg/ml)
$425
Description
Myeloperoxidase antibody LS-B6695 is an unconjugated rabbit polyclonal antibody to human Myeloperoxidase (MPO). Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human MPO / Myeloperoxidase
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • IHC - Frozen
  • Immunofluorescence
  • Western blot (10 - 50 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Native myeloperoxidase isolated from human leucocytes.
Specificity
Specific for MPO.
Usage
IHC: This antibody performs superbly in paraffin-embedded tissue sections fixed in formalin, frozen sections and cell cytospins. Antigen retrieval is essential for use on paraffin sections.
Presentation
Lyophilized
Reconstitution
500 µl Sterile water
Storage
Maintain lyophilized and reconstituted antibodies at -20°C for long term storage and at 2°C to 8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About MPO / Myeloperoxidase
P05164 NM_000250 NP_000241.1

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Images

Immunohistochemistry

Anti-MPO / Myeloperoxidase antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-MPO / Myeloperoxidase antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

Immunofluorescence

Rabbit antibody to MPO. Confocal microscopy on isolated monocytes. MPO detected with Rabbit antibody to MPO: red; Hoechst: blue; MHC class II: green. Magnification: 200 X
Rabbit antibody to MPO. Confocal microscopy on isolated monocytes. MPO detected with Rabbit antibody to MPO: red; Hoechst: blue; MHC class II: green. Magnification: 200 X

Immunofluorescence

Rabbit antibody to MPO. Isolated monocytes were stained with Lysotrack red followed by staining with Rabbit antibody to MPO (3ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analyzed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 200X
Rabbit antibody to MPO. Isolated monocytes were stained with Lysotrack red followed by staining with Rabbit antibody to MPO (3ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analyzed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 200X

Western blot

Rabbit antibody to MPO. 10 ug of neutrophil lysate was separated by 12% SDS-PAGE. Proteins were transferred onto a PVDF membrane and blocked by incubation with PBS containing 2% skim milk and 0.02% Tween 20 for 30 min. Membranes were probed with Rabbit antibody to MPO (3 ug/ml) for 1 hr. Membranes were then probed with goat anti-rabbit antibody conjugated to alkaline phosphatase diluted 1:1000 and developed with ECF luminescence substrate (Amersham Biosciences).
Rabbit antibody to MPO. 10 ug of neutrophil lysate was separated by 12% SDS-PAGE. Proteins were transferred onto a PVDF membrane and blocked by incubation with PBS containing 2% skim milk and 0.02% Tween 20 for 30 min. Membranes were probed with Rabbit antibody to MPO (3 ug/ml) for 1 hr. Membranes were then probed with goat anti-rabbit antibody conjugated to alkaline phosphatase diluted 1:1000 and developed with ECF luminescence substrate (Amersham Biosciences).

Immunofluorescence

Rabbit antibody to MPO. Isolated monocytes were stained with red followed by staining with Rabbit antibody (3 ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analyzed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 80X
Rabbit antibody to MPO. Isolated monocytes were stained with red followed by staining with Rabbit antibody (3 ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analyzed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 80X

Immunohistochemistry

Anti-MPO / Myeloperoxidase antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-MPO / Myeloperoxidase antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

Immunofluorescence

Rabbit antibody to MPO. Confocal microscopy on isolated monocytes. MPO detected with Rabbit antibody to MPO: red; Hoechst: blue; MHC class II: green. Magnification: 200 X
Rabbit antibody to MPO. Confocal microscopy on isolated monocytes. MPO detected with Rabbit antibody to MPO: red; Hoechst: blue; MHC class II: green. Magnification: 200 X

Immunofluorescence

Rabbit antibody to MPO. Isolated monocytes were stained with Lysotrack red followed by staining with Rabbit antibody to MPO (3ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analyzed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 200X
Rabbit antibody to MPO. Isolated monocytes were stained with Lysotrack red followed by staining with Rabbit antibody to MPO (3ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analyzed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 200X

Western blot

Rabbit antibody to MPO. 10 ug of neutrophil lysate was separated by 12% SDS-PAGE. Proteins were transferred onto a PVDF membrane and blocked by incubation with PBS containing 2% skim milk and 0.02% Tween 20 for 30 min. Membranes were probed with Rabbit antibody to MPO (3 ug/ml) for 1 hr. Membranes were then probed with goat anti-rabbit antibody conjugated to alkaline phosphatase diluted 1:1000 and developed with ECF luminescence substrate (Amersham Biosciences).
Rabbit antibody to MPO. 10 ug of neutrophil lysate was separated by 12% SDS-PAGE. Proteins were transferred onto a PVDF membrane and blocked by incubation with PBS containing 2% skim milk and 0.02% Tween 20 for 30 min. Membranes were probed with Rabbit antibody to MPO (3 ug/ml) for 1 hr. Membranes were then probed with goat anti-rabbit antibody conjugated to alkaline phosphatase diluted 1:1000 and developed with ECF luminescence substrate (Amersham Biosciences).

Immunofluorescence

Rabbit antibody to MPO. Isolated monocytes were stained with red followed by staining with Rabbit antibody (3 ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analyzed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 80X
Rabbit antibody to MPO. Isolated monocytes were stained with red followed by staining with Rabbit antibody (3 ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analyzed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 80X

Immunohistochemistry

Anti-MPO / Myeloperoxidase antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-MPO / Myeloperoxidase antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

Immunofluorescence

Rabbit antibody to MPO. Confocal microscopy on isolated monocytes. MPO detected with Rabbit antibody to MPO: red; Hoechst: blue; MHC class II: green. Magnification: 200 X
Rabbit antibody to MPO. Confocal microscopy on isolated monocytes. MPO detected with Rabbit antibody to MPO: red; Hoechst: blue; MHC class II: green. Magnification: 200 X

Immunofluorescence

Rabbit antibody to MPO. Isolated monocytes were stained with Lysotrack red followed by staining with Rabbit antibody to MPO (3ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analyzed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 200X
Rabbit antibody to MPO. Isolated monocytes were stained with Lysotrack red followed by staining with Rabbit antibody to MPO (3ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analyzed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 200X

Western blot

Rabbit antibody to MPO. 10 ug of neutrophil lysate was separated by 12% SDS-PAGE. Proteins were transferred onto a PVDF membrane and blocked by incubation with PBS containing 2% skim milk and 0.02% Tween 20 for 30 min. Membranes were probed with Rabbit antibody to MPO (3 ug/ml) for 1 hr. Membranes were then probed with goat anti-rabbit antibody conjugated to alkaline phosphatase diluted 1:1000 and developed with ECF luminescence substrate (Amersham Biosciences).
Rabbit antibody to MPO. 10 ug of neutrophil lysate was separated by 12% SDS-PAGE. Proteins were transferred onto a PVDF membrane and blocked by incubation with PBS containing 2% skim milk and 0.02% Tween 20 for 30 min. Membranes were probed with Rabbit antibody to MPO (3 ug/ml) for 1 hr. Membranes were then probed with goat anti-rabbit antibody conjugated to alkaline phosphatase diluted 1:1000 and developed with ECF luminescence substrate (Amersham Biosciences).

Immunofluorescence

Rabbit antibody to MPO. Isolated monocytes were stained with red followed by staining with Rabbit antibody (3 ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analyzed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 80X
Rabbit antibody to MPO. Isolated monocytes were stained with red followed by staining with Rabbit antibody (3 ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analyzed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 80X

Immunohistochemistry

Anti-MPO / Myeloperoxidase antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-MPO / Myeloperoxidase antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

Immunofluorescence

Rabbit antibody to MPO. Confocal microscopy on isolated monocytes. MPO detected with Rabbit antibody to MPO: red; Hoechst: blue; MHC class II: green. Magnification: 200 X
Rabbit antibody to MPO. Confocal microscopy on isolated monocytes. MPO detected with Rabbit antibody to MPO: red; Hoechst: blue; MHC class II: green. Magnification: 200 X

Immunofluorescence

Rabbit antibody to MPO. Isolated monocytes were stained with Lysotrack red followed by staining with Rabbit antibody to MPO (3ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analyzed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 200X
Rabbit antibody to MPO. Isolated monocytes were stained with Lysotrack red followed by staining with Rabbit antibody to MPO (3ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analyzed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 200X

Western blot

Rabbit antibody to MPO. 10 ug of neutrophil lysate was separated by 12% SDS-PAGE. Proteins were transferred onto a PVDF membrane and blocked by incubation with PBS containing 2% skim milk and 0.02% Tween 20 for 30 min. Membranes were probed with Rabbit antibody to MPO (3 ug/ml) for 1 hr. Membranes were then probed with goat anti-rabbit antibody conjugated to alkaline phosphatase diluted 1:1000 and developed with ECF luminescence substrate (Amersham Biosciences).
Rabbit antibody to MPO. 10 ug of neutrophil lysate was separated by 12% SDS-PAGE. Proteins were transferred onto a PVDF membrane and blocked by incubation with PBS containing 2% skim milk and 0.02% Tween 20 for 30 min. Membranes were probed with Rabbit antibody to MPO (3 ug/ml) for 1 hr. Membranes were then probed with goat anti-rabbit antibody conjugated to alkaline phosphatase diluted 1:1000 and developed with ECF luminescence substrate (Amersham Biosciences).

Immunofluorescence

Rabbit antibody to MPO. Isolated monocytes were stained with red followed by staining with Rabbit antibody (3 ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analyzed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 80X
Rabbit antibody to MPO. Isolated monocytes were stained with red followed by staining with Rabbit antibody (3 ug/ml) for 1 hour at room temperature, washed and followed by staining with goat anti-rabbit antibody conjugated to Alexa 488 (Green) for 1 hr. Mounting media (10% Glycerol in PBS) containing 33258 1 ug/ml (Sigma-Aldrich) was used for nuclear counterstaining (Blue). Fluorescent cell staining was analyzed by confocal microscopy using a BioRad Radiance 2100 confocal microscope. Magnification: 80X

Requested From: United States
Date Requested: 12/18/2018
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