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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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MPO / Myeloperoxidase Antibody IHC‑plus™ LS‑B392
Note: This antibody replaces LS-C19072
Myeloperoxidase antibody LS-B392 is an unconjugated rabbit polyclonal antibody to human Myeloperoxidase (MPO). Validated for ELISA, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B392-50
50 µl (85 mg/ml)
$425
Description
Myeloperoxidase antibody LS-B392 is an unconjugated rabbit polyclonal antibody to human Myeloperoxidase (MPO). Validated for ELISA, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human MPO / Myeloperoxidase
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:500)
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Specificity
Myeloperoxidase [Human Leukocytes].
Usage
Immunohistochemistry: LS-B392 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B392 was determined to be 1:500.
Presentation
PBS, pH 7.2, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About MPO / Myeloperoxidase
P05164 NM_000250 NP_000241.1

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Images

Immunohistochemistry

Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

Western blot

Anti-Myeloperoxidase (Human White Blood Cell) Antibody - Western Blot. Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kD and 15 kD polypeptides and chain combinations forming 68 kD and 106 kD proteins. The staining of the 68 kD band is so intense that is over saturates the signal detection. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 2 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Anti-Myeloperoxidase (Human White Blood Cell) Antibody - Western Blot. Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kD and 15 kD polypeptides and chain combinations forming 68 kD and 106 kD proteins. The staining of the 68 kD band is so intense that is over saturates the signal detection. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 2 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Western blot

Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot.  Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combinations forming 68 kDa and 106 kDa proteins.  The staining of the 68 kDa band is so intense that is over saturates the signal detection.
Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combinations forming 68 kDa and 106 kDa proteins. The staining of the 68 kDa band is so intense that is over saturates the signal detection.

Immunohistochemistry

Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

Western blot

Anti-Myeloperoxidase (Human White Blood Cell) Antibody - Western Blot. Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kD and 15 kD polypeptides and chain combinations forming 68 kD and 106 kD proteins. The staining of the 68 kD band is so intense that is over saturates the signal detection. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 2 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Anti-Myeloperoxidase (Human White Blood Cell) Antibody - Western Blot. Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kD and 15 kD polypeptides and chain combinations forming 68 kD and 106 kD proteins. The staining of the 68 kD band is so intense that is over saturates the signal detection. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 2 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Western blot

Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot.  Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combinations forming 68 kDa and 106 kDa proteins.  The staining of the 68 kDa band is so intense that is over saturates the signal detection.
Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combinations forming 68 kDa and 106 kDa proteins. The staining of the 68 kDa band is so intense that is over saturates the signal detection.

Immunohistochemistry

Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

Western blot

Anti-Myeloperoxidase (Human White Blood Cell) Antibody - Western Blot. Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kD and 15 kD polypeptides and chain combinations forming 68 kD and 106 kD proteins. The staining of the 68 kD band is so intense that is over saturates the signal detection. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 2 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Anti-Myeloperoxidase (Human White Blood Cell) Antibody - Western Blot. Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kD and 15 kD polypeptides and chain combinations forming 68 kD and 106 kD proteins. The staining of the 68 kD band is so intense that is over saturates the signal detection. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 2 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Western blot

Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot.  Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combinations forming 68 kDa and 106 kDa proteins.  The staining of the 68 kDa band is so intense that is over saturates the signal detection.
Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combinations forming 68 kDa and 106 kDa proteins. The staining of the 68 kDa band is so intense that is over saturates the signal detection.

Immunohistochemistry

Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-MPO / Myeloperoxidase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

Western blot

Anti-Myeloperoxidase (Human White Blood Cell) Antibody - Western Blot. Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kD and 15 kD polypeptides and chain combinations forming 68 kD and 106 kD proteins. The staining of the 68 kD band is so intense that is over saturates the signal detection. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 2 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Anti-Myeloperoxidase (Human White Blood Cell) Antibody - Western Blot. Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kD and 15 kD polypeptides and chain combinations forming 68 kD and 106 kD proteins. The staining of the 68 kD band is so intense that is over saturates the signal detection. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 2 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Western blot

Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot.  Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combinations forming 68 kDa and 106 kDa proteins.  The staining of the 68 kDa band is so intense that is over saturates the signal detection.
Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combinations forming 68 kDa and 106 kDa proteins. The staining of the 68 kDa band is so intense that is over saturates the signal detection.

Requested From: United States
Date Requested: 12/13/2018
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