Human MPO / Myeloperoxidase
(tested or 100% immunogen sequence identity)
Protein G purified
Recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55kD) and two light chains (15kD), the heavy chains being linked by a disulphide bond. Recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. Also recognizes recombinant MPO in Western blots and weakly in ELISA. May be of value in the study of myeloid cells and myeloid leukemias by flow cytometry following cell permeablization.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, 0.09% Sodium Azide, 5% Sucrose, 1% BSA
Reconstitute with distilled water.
Store at 4°C. Do not freeze. Store undiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.
For research use only.
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About MPO / Myeloperoxidase