(tested or 100% immunogen sequence identity)
Also available Unconjugated
or conjugated with FITC
- IHC - Paraffin
- Dot Blot
- Applications tested for the base form of this product only
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Highly purified normal homogenous IgG2a(1b) isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Mouse IgG2a(1b), allotype specific.
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is restricted to the Fc portion of the IgG2a molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against mouse serum of the allelic type Igh-1b, including C57Bl and SJL/J, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG2a(1b). No precipitation reaction is obtained with purified IgG2a(1a) (Balb/C, CBA, AKR and NZB), other IgG subclasses, IgA, IgG/Fab fragments or other serum proteins. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG2a(1b) at the cellular an subcellular level. In non-isotopic assay methodology (e. g. ELISA and Western blotting) to identify and measure IgG2a(1b) in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. Its optimum working dilution should be established by titration. Excess labeled antibody may by itself cause high unspecific background staining or interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:200. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:2000. These data should be interpreted as general recommendations only.
Lyophilized from PBS, pH 7.2, No preservatives added
1 ml Distilled water
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.