Mouse IgG2a[b]

Sheep

Mouse (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with FITC, Biotin, HRP.

Purified IgG

Unmodified

Highly purified normal homogenous IgG2a(1b) isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Mouse IgG2a(1b), allotype specific.

• Immunofluorescence
• ELISA
• Dot Blot

The reactivity of the antiserum is restricted to the Fc portion of the IgG2a molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against mouse serum of the allelic type Igh-1b, including C57Bl and SJL/J, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG2a(1b). No precipitation reaction is obtained with purified IgG2a(1a) (Balb/C, CBA, AKR and NZB), other IgG subclasses, IgA, IgG/Fab fragments or other serum proteins. As unlabelled primary or secondary reagent for indirect detection of IgG2a(1b) at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. to prepare conjugates of the user's own choice. to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Typical working dilutions in histochemistry are usually between 1:100 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:4.000. These data should be interpreted as general recommendations only.

Lyophilized from PBS, pH 7.2, No preservatives added

Reconstitute with distilled water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.