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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C347129-10 10 mg $475 
Antibody

Mouse IgG2a[b] Antibody LS‑C347129

Mouse IgG2a[b] Antibody LS‑C347129

Antibody:
Sheep Polyclonal to Mouse Mouse IgG2a[b]
Application:
IF, ELISA, DB
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$475 
LS-C347129-10
10 mg
Toll Free North America
866-819-4732

Overview

Antibody:
Sheep Polyclonal to Mouse Mouse IgG2a[b]
Application:
IF, ELISA, DB
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Mouse IgG2a[b] antibody LS-C347129 is an unconjugated sheep polyclonal antibody to mouse Mouse IgG2a[b]. Validated for DB, ELISA and IF.
Target
Mouse IgG2a[b]
Host
Sheep
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Purification
Purified IgG
Modifications
Unmodified
Applications
  • Immunofluorescence
  • ELISA
  • Dot Blot
  • Applications tested for the base form of this product only
Immunogen
Highly purified normal homogenous IgG2a(1b) isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Mouse IgG2a(1b), allotype specific.
Usage
The reactivity of the antiserum is restricted to the Fc portion of the IgG2a molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against mouse serum of the allelic type Igh-1b, including C57Bl and SJL/J, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG2a(1b). No precipitation reaction is obtained with purified IgG2a(1a) (Balb/C, CBA, AKR and NZB), other IgG subclasses, IgA, IgG/Fab fragments or other serum proteins. As unlabelled primary or secondary reagent for indirect detection of IgG2a(1b) at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. to prepare conjugates of the user's own choice. to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Typical working dilutions in histochemistry are usually between 1:100 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:4.000. These data should be interpreted as general recommendations only.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
Reconstitute with distilled water.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 10/15/2019