Isotype Control

Mouse

IgG2a Monoclonal

OX-34

FITC

Protein G purified

Unmodified

Activated rat T-helper cells.

Suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue. May not be suitable for intracellular staining on some cell types. Clone MRC OX-34 recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species. This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control. Test results have shown that the antibody is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.

• Flow Cytometry

It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test reagent. Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PBS, 0.09% Sodium Azide, 1% BSA

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Protect from light. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.