Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone R1-3G1] (IgG1,k) to Rat IgG1
Rat (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [R1-3G1]
Specificity and Use
Rat IgG1 antibody was raised against rat IgG1
This R1-3G1 antibody has been tested by flow cytometric analysis of mouse splenocytes stained with rat IgG1 monoclonal antibodies. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, 0.09% sodium azide
Store at +4°C. Do not freeze. Product is photosensitive and should be protected from light.
3-day Concanavalin A-stimulated C57BL/6 splenocytes were stained with staining buffer alone (red line), or purified rat IgG1 anti-mouse CD25, clone PC61.5, followed by FITC rat polyclonal anti-rat IgG (blue line) or isotype specific FITC anti-rat IgG1, clone R1-3G1 (green line). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.