Human IgA2

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Biotin. Also available Unconjugated or conjugated with HRP, TRITC, FITC.

Unmodified

• IHC - Paraffin
• ICC
• Western blot
• ELISA
• Dot Blot
• Applications tested for the base form of this product only

The reactivity of the antiserum is restricted to an allotype specific determinant on IgA2(m)2 molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgA2(m)2, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used. The optimum working dilution is an assayrelated characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:200 upwards; in Western blotting from 1:200 upwards.

PBS, pH 7.2

Reconstitute with 0.5ml sterile distilled water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.