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Antibodies > DYKDDDDK Tag > LS-C203597
 
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Catalog Number Size Price
LS-C203597-100 100 µg $334 
LS-C203597-200 200 µg $436 
DYKDDDDK Tag Antibody - 293 cells transfected with C-terminal DYKDDDDK tag protein. Primary antibody: 1 ?g/ml THETM Anti-DYKDDDDK-tag Monoclonal Antibody (Mouse). Secondary antibody: 2 ?g/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121).
DYKDDDDK Tag Antibody - 293 cells transfected with N-terminal DYKDDDDK tag protein. Primary antibody: 1 ug/ml THETM Anti-DYKDDDDK-tag Monoclonal Antibody (Mouse). Secondary antibody: 2 ug/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121).
DYKDDDDK Tag Antibody - Western blot of E. coli lysate containing DYKDDDDK-tagged fusion protein and its immunoprecipitates. 1: E. coli lysate containing DYKDDDDK-tagged fusion protein. 2: Immunoprecipitates of free E. coli lysate incubated with DYKDDDDK Tag Antibody, mAb, Mouse (2 ug/ml) and Protein A. 3: Immunoprecipitates of E. coli lysate containing DYKDDDDK-tagged fusion protein incubated with DYKDDDDK Tag Antibody, mAb, Mouse (2 ug/ml) and Protein A.
DYKDDDDK Tag Antibody - Consistency analysis of Batch 1# and 2# of THETM DYKDDDDK Tag Antibody, mAb, Mouse (1 ug/ml) by Western blot, showing that signal remains consistent from Lot to Lot. The assay was performed with DYKDDDDK-tagged fusion protein. The signal was developed with IRDye 800 Conjugated Goat Anti-Mouse IgG.
DYKDDDDK Tag Antibody - Comparison of DYKDDDDK Tag Antibody, mAb, Mouse (A: 1 ug/ml) with Mouse Anti- DYKDDDDK Tag mAb (B: Company S, clone M2, 1 ug/ml) by Western blot. Lane 1 N-terminal DYKDDDDK-tagged fusion protein. Lane 2 Internal DYKDDDDK-tagged fusion protein. Lane 3 C-terminal DYKDDDDK-tagged fusion protein. Predicted Size: N-terminal DYKDDDDK-tagged fusion protein 52 kD. Internal DYKDDDDK-tagged fusion protein 55 kD. C-terminal DYKDDDDK-tagged fusion protein 55 kD. Observed Size: N-terminal DYKDDDDK-tagged fusion protein 52 kD. Internal DYKDDDDK-tagged fusion protein 55 kD. C-terminal DYKDDDDK-tagged fusion protein 55 kD. The signal was developed with IRDye 800 Conjugated Goat Anti-Mouse IgG.
DYKDDDDK Tag Antibody - Loading: Lane 1-3 Multiple Tag (Purified) (400 ng, 80 ng,16 ng). Lane 4. 293 cell lysate 10 ul. Primary antibody: A. 1 ug/ml THETM Mouse Anti-DYKDDDDK-tag Monoclonal Antibody). B. 1 ug/ml Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (Company S, clone M2). Secondary antibody: IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG (ROCKLAND, 1: 10000,610-132-121).
DYKDDDDK Tag Antibody - 293 cells transfected with C-terminal DYKDDDDK tag protein. Primary antibody: 1 ?g/ml THETM Anti-DYKDDDDK-tag Monoclonal Antibody (Mouse). Secondary antibody: 2 ?g/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121).
DYKDDDDK Tag Antibody - 293 cells transfected with N-terminal DYKDDDDK tag protein. Primary antibody: 1 ug/ml THETM Anti-DYKDDDDK-tag Monoclonal Antibody (Mouse). Secondary antibody: 2 ug/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121).
DYKDDDDK Tag Antibody - Western blot of E. coli lysate containing DYKDDDDK-tagged fusion protein and its immunoprecipitates. 1: E. coli lysate containing DYKDDDDK-tagged fusion protein. 2: Immunoprecipitates of free E. coli lysate incubated with DYKDDDDK Tag Antibody, mAb, Mouse (2 ug/ml) and Protein A. 3: Immunoprecipitates of E. coli lysate containing DYKDDDDK-tagged fusion protein incubated with DYKDDDDK Tag Antibody, mAb, Mouse (2 ug/ml) and Protein A.
DYKDDDDK Tag Antibody - Consistency analysis of Batch 1# and 2# of THETM DYKDDDDK Tag Antibody, mAb, Mouse (1 ug/ml) by Western blot, showing that signal remains consistent from Lot to Lot. The assay was performed with DYKDDDDK-tagged fusion protein. The signal was developed with IRDye 800 Conjugated Goat Anti-Mouse IgG.
DYKDDDDK Tag Antibody - Comparison of DYKDDDDK Tag Antibody, mAb, Mouse (A: 1 ug/ml) with Mouse Anti- DYKDDDDK Tag mAb (B: Company S, clone M2, 1 ug/ml) by Western blot. Lane 1 N-terminal DYKDDDDK-tagged fusion protein. Lane 2 Internal DYKDDDDK-tagged fusion protein. Lane 3 C-terminal DYKDDDDK-tagged fusion protein. Predicted Size: N-terminal DYKDDDDK-tagged fusion protein 52 kD. Internal DYKDDDDK-tagged fusion protein 55 kD. C-terminal DYKDDDDK-tagged fusion protein 55 kD. Observed Size: N-terminal DYKDDDDK-tagged fusion protein 52 kD. Internal DYKDDDDK-tagged fusion protein 55 kD. C-terminal DYKDDDDK-tagged fusion protein 55 kD. The signal was developed with IRDye 800 Conjugated Goat Anti-Mouse IgG.
DYKDDDDK Tag Antibody - Loading: Lane 1-3 Multiple Tag (Purified) (400 ng, 80 ng,16 ng). Lane 4. 293 cell lysate 10 ul. Primary antibody: A. 1 ug/ml THETM Mouse Anti-DYKDDDDK-tag Monoclonal Antibody). B. 1 ug/ml Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (Company S, clone M2). Secondary antibody: IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG (ROCKLAND, 1: 10000,610-132-121).
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Monoclonal Mouse DYKDDDDK Tag Antibody (clone 5A8E5, IF, WB) LS‑C203597

Monoclonal Mouse DYKDDDDK Tag Antibody (clone 5A8E5, IF, WB) LS‑C203597

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Antibody:
DYKDDDDK Tag Mouse Monoclonal (5A8E5) Antibody
Application:
IF, WB, IP, ELISA
Format:
Unconjugated, Unmodified
Price
Catalog Number
$334
LS-C203597-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DYKDDDDK Tag Mouse Monoclonal (5A8E5) Antibody
Application:
IF, WB, IP, ELISA
Format:
Unconjugated, Unmodified

Specifications

Description
DYKDDDDK Tag antibody LS-C203597 is an unconjugated mouse monoclonal antibody to DYKDDDDK Tag. Validated for ELISA, IF, IP and WB.
Target
DYKDDDDK Tag
Host
Mouse
Clonality
IgG2b,k Monoclonal
Clone
5A8E5
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation (1 µg/ml)
  • ELISA (0.05 - 0.2 µg/ml)
Presentation
Lyophilized from PBS, pH 7.4, 0.02% Sodium Azide
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Storage
Store at -20°C. Long term: aliquot and store at -20°C. Reconstituted: store at 2°C to 8°C for up to 3 weeks. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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DYKDDDDK Tag Antibody - Western Blot of Mouse antibody for the detection of FLAG™ conjugated proteins Monoclonal Antibody Peroxidase Conjugated. Lane 1: FLAG™ Positive Control Lysates. Load: 10 µg per lane. Primary antibody: FLAG™ Monoclonal Antibody Peroxidase Conjugated at 1:1,000 for overnight at 4°C. Secondary antibody: none.
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DYKDDDDK Tag Antibody - Immunofluorescent analysis of FLAG-tag staining in 293T cells transfected with a Flag-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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DYKDDDDK Tag Antibody - Antibody for the detection of FLAG conjugated proteins - Western Blot. Affinity Purified Antibody to detect FLAG conjugated proteins detects both C terminal linked and N terminal linked FLAG tagged recombinant proteins by western blot. This antibody was used at a dilution of 1:2500 to detect 1.0 ug of recombinant protein containing either the FLAG epitope tag linked at the carboxy (C) or the amino (N) terminus of the recombinant protein. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
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