Duck IgY

Mouse

Duck (tested or 100% immunogen sequence identity)

Chicken

IgG1 Monoclonal

14A3

Unconjugated

Affinity purified

Unmodified

Purified Peking duck yolk IgY.

Recognizes the light chain of duck immunoglobulin, shown to be expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes. Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1, revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+ /14A3+ cells in duck bursa. The recognition of B cells by 14A3 was confirmed using a recently developed Flag-tagged chicken B cell marker, known as chBAFF-Flag (B cell activating factor). Recognizes IgA in Peking duck bile and can be used as a marker to identify B cells in both Peking and Muscovy duck and geese studies. Detects a band of approximately 25 kD in duck cell lysates in Western blotting.

• Western blot
• Flow Cytometry
• ELISA

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

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