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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MNAT1 Antibody (Internal) LS‑C352528
MNAT1 antibody LS-C352528 is an unconjugated rabbit polyclonal antibody to MNAT1 from human, mouse, rat and other species. Validated for IHC, IP and WB.
Catalog
Size
Price
LS-C352528-100
100 µl (1 mg/ml)
$245

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Product Description

MNAT1 Antibody (Internal) for IHC, WB/Western, IP LS-C352528

Specifications

Target
Human MNAT1
Synonyms
MNAT1, Cyclin G1 interacting protein, Cyclin-G1-interacting protein, MAT1, p35, p36, RING finger protein 66, RING finger protein MAT1, Menage a trois, RNF66, TFB3, CAP35, CDK7/cyclin-H assembly factor
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MNAT1 antibody was raised against kLH-conjugated synthetic peptide encompassing a sequence within the central region of human MNAT1.
Blocking Peptide
LS-E40932 - Lyophilized - 1 mg - $145.00
Immunizing peptide used to generate LS-C352528. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Internal
Specificity
Recognizes endogenous levels of MNAT1 protein.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
About MNAT1
Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. P51948 NM_002431 NP_002422.1


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Images

Immunohistochemistry

Immunohistochemical analysis of MNAT1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of MNAT1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Western blot

Western blot analysis of MNAT1 expression in HeLa (A); MCF7 (B); A431 (C); NIH3T3 (D) whole cell lysates.
Western blot analysis of MNAT1 expression in HeLa (A); MCF7 (B); A431 (C); NIH3T3 (D) whole cell lysates.

Immunohistochemistry

Immunohistochemical analysis of MNAT1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of MNAT1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Western blot

Western blot analysis of MNAT1 expression in HeLa (A); MCF7 (B); A431 (C); NIH3T3 (D) whole cell lysates.
Western blot analysis of MNAT1 expression in HeLa (A); MCF7 (B); A431 (C); NIH3T3 (D) whole cell lysates.

Immunohistochemistry

Immunohistochemical analysis of MNAT1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of MNAT1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Western blot

Western blot analysis of MNAT1 expression in HeLa (A); MCF7 (B); A431 (C); NIH3T3 (D) whole cell lysates.
Western blot analysis of MNAT1 expression in HeLa (A); MCF7 (B); A431 (C); NIH3T3 (D) whole cell lysates.

Immunohistochemistry

Immunohistochemical analysis of MNAT1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of MNAT1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Western blot

Western blot analysis of MNAT1 expression in HeLa (A); MCF7 (B); A431 (C); NIH3T3 (D) whole cell lysates.
Western blot analysis of MNAT1 expression in HeLa (A); MCF7 (B); A431 (C); NIH3T3 (D) whole cell lysates.

Requested From: United States
Date Requested: 10/16/2018

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