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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MNAT1 Antibody (aa92‑278) LS‑C407827
MNAT1 antibody LS-C407827 is an unconjugated rabbit polyclonal antibody to MNAT1 from human, mouse and rat. Validated for IHC and WB.
Catalog
Size
Price
LS-C407827-100
100 µg
$355

Popular MNAT1 Products

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Immunohistochemical analysis of MNAT1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Product Description

MNAT1 antibody LS-C407827 is an unconjugated rabbit polyclonal antibody to MNAT1 from human, mouse and rat. Validated for IHC and WB.
About MNAT1
Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. P51948 NM_002431 NP_002422.1

MNAT1 Antibody, Cyclin G1 interacting protein Antibody, Cyclin-G1-interacting protein Antibody, MAT1 Antibody, p35 Antibody, p36 Antibody, RING finger protein 66 Antibody, RING finger protein MAT1 Antibody, Menage a trois Antibody, RNF66 Antibody, TFB3 Antibody, CAP35 Antibody, CDK7/cyclin-H assembly factor Antibody


Specifications

Target
Human MNAT1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MNAT1 antibody was raised against e.coli-derived human MNAT1 recombinant protein (Position: L92-A278). Human MNAT1 shares 93% amino acid (aa) sequence identity with mouse MNAT1.
Epitope
aa92-278
Specificity
Highest levels in colon and testis. Moderate levels are present thymus, prostate, ovary, and small intestine. The lowest levels are found in spleen and leukocytes.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 7.1 mM sodium phosphate, 77 mM NaCl, 2.5% BSA, 0.025% sodium azide
Reconstitution
Reconstitute with 200 µl of distilled water.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

MNAT1 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.
MNAT1 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.

Immunohistochemistry

MNAT1 antibody IHC-paraffin. IHC(P): Human Testis Tissue.
MNAT1 antibody IHC-paraffin. IHC(P): Human Testis Tissue.

Immunohistochemistry

MNAT1 antibody IHC-paraffin. IHC(P): Mouse Testis Tissue.
MNAT1 antibody IHC-paraffin. IHC(P): Mouse Testis Tissue.

Western blot

MNAT1 antibody Western blot. All lanes: Anti MNAT1 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: MCF-7 Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Predicted band size: 81 kD. Observed band size: 81 kD.
MNAT1 antibody Western blot. All lanes: Anti MNAT1 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: MCF-7 Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Predicted band size: 81 kD. Observed band size: 81 kD.

Immunohistochemistry

MNAT1 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.
MNAT1 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.

Immunohistochemistry

MNAT1 antibody IHC-paraffin. IHC(P): Human Testis Tissue.
MNAT1 antibody IHC-paraffin. IHC(P): Human Testis Tissue.

Immunohistochemistry

MNAT1 antibody IHC-paraffin. IHC(P): Mouse Testis Tissue.
MNAT1 antibody IHC-paraffin. IHC(P): Mouse Testis Tissue.

Western blot

MNAT1 antibody Western blot. All lanes: Anti MNAT1 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: MCF-7 Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Predicted band size: 81 kD. Observed band size: 81 kD.
MNAT1 antibody Western blot. All lanes: Anti MNAT1 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: MCF-7 Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Predicted band size: 81 kD. Observed band size: 81 kD.

Immunohistochemistry

MNAT1 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.
MNAT1 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.

Immunohistochemistry

MNAT1 antibody IHC-paraffin. IHC(P): Human Testis Tissue.
MNAT1 antibody IHC-paraffin. IHC(P): Human Testis Tissue.

Immunohistochemistry

MNAT1 antibody IHC-paraffin. IHC(P): Mouse Testis Tissue.
MNAT1 antibody IHC-paraffin. IHC(P): Mouse Testis Tissue.

Western blot

MNAT1 antibody Western blot. All lanes: Anti MNAT1 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: MCF-7 Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Predicted band size: 81 kD. Observed band size: 81 kD.
MNAT1 antibody Western blot. All lanes: Anti MNAT1 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: MCF-7 Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Predicted band size: 81 kD. Observed band size: 81 kD.

Immunohistochemistry

MNAT1 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.
MNAT1 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.

Immunohistochemistry

MNAT1 antibody IHC-paraffin. IHC(P): Human Testis Tissue.
MNAT1 antibody IHC-paraffin. IHC(P): Human Testis Tissue.

Immunohistochemistry

MNAT1 antibody IHC-paraffin. IHC(P): Mouse Testis Tissue.
MNAT1 antibody IHC-paraffin. IHC(P): Mouse Testis Tissue.

Western blot

MNAT1 antibody Western blot. All lanes: Anti MNAT1 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: MCF-7 Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Predicted band size: 81 kD. Observed band size: 81 kD.
MNAT1 antibody Western blot. All lanes: Anti MNAT1 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: MCF-7 Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Predicted band size: 81 kD. Observed band size: 81 kD.

Requested From: United States
Date Requested: 8/16/2018

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