Human MLANA / Melan-A

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein G purified

Azide-free

Recombinant MART-1 protein.

Recognizes MART-1 at ~18kD. Does not cross-react with MAGE-1 or tyrosinase protein. Species Reactivity: Human.

• IHC
• IHC - Paraffin
• IHC - Frozen
• Western blot (1 µg/ml)
• Immunoprecipitation

Suitable for use in Immunoprecipitation, Western Blot, and Immunohistochemistry (frozen & formalin/paraffin). Immunoprecipitation: Use Protein G; 2 ug antibody/mg protein lysate. Western Blot: 1 ug/ml for 2 hours at RT. Immunohistochemistry: 1-2 ug/ml for 30 min. at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min. Positive control: CaC1 melanoma cells and melanomas. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

10 mM PBS, pH 7.4

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q16655 NCBI: NM_005511 NP_005502.1