Human Mitotic Cells
(tested or 100% immunogen sequence identity)
- ICC (1:50 - 1:100)
- Flow Cytometry (1:50 - 1:100)
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- IHC - Paraffin
- Western blot
Total cell lysate of the human bladder carcinoma cell line T24
Strongly stains mitotic cells.
Strongly stains mitotic cells and can therefore be used in flow cytometric analyses of cell suspensions to detect the mitotic index. Together with a quantitative DNA staining procedure (e.g. propidium iodide) antibody clearly distinguishes these M-phase cells from cell at other stages of the cell cycle. Dynamic information can be obtained by combining BrdU incorporation with antibody staining, which can distinguish and quantitate the four major fractions of the cell cycle.
PBS, 0.09% Sodium Azide
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
For research use only.
This antibody carries the LSBio 100% Guarantee.