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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MICA/B Antibody LS-C44797

Note: This antibody replaces LS-C57932
B antibody LS-C44797 is an unconjugated mouse monoclonal antibody to human B (MICA). Validated for Flow, IHC and IP.
0.2 mg
B antibody LS-C44797 is an unconjugated mouse monoclonal antibody to human B (MICA). Validated for Flow, IHC and IP.
Human MICA/B
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal
Unconjugated. Also available conjugated with FITC.
Protein G purified
Unmodified. Also available Low Endotoxin.
  • IHC
  • IHC - Frozen (1:100)
  • Immunoprecipitation
  • Flow Cytometry
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
MICA/B antibody was raised against mICA transfected C1R cells.
Specific for nonclassical MHC class I chain A (MICA) and nonclassical MHC class I chain MICB (MICB). MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. MICA is principally expressed on intestinal epithelium, and several epithelial tumors. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection. Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells. XXX recommend the use of MCA2304EL for such studies.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. Method sheets are available on request. Membrane permeabilization is required for this application.
PBS, pH 7.4, 0.09% Sodium Azide
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.

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Staining of HeLa cells with PE Mouse IgG2a, K isotype control (open histogram) or PE anti-human MICA/MICB (6D4) (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Requested From: United States
Date Requested: 3/26/2019
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