B antibody LS-C44797 is an unconjugated mouse monoclonal antibody to human B (MICA). Validated for Flow, IHC and IP.
(tested or 100% immunogen sequence identity)
Also available conjugated with FITC
Protein G purified
Also available Low Endotoxin
- IHC - Frozen (1:100)
- Flow Cytometry
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
MICA/B antibody was raised against mICA transfected C1R cells.
Specific for nonclassical MHC class I chain A (MICA) and nonclassical MHC class I chain MICB (MICB). MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. MICA is principally expressed on intestinal epithelium, and several epithelial tumors. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection. Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells. XXX recommend the use of MCA2304EL for such studies.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. Method sheets are available on request. Membrane permeabilization is required for this application.
PBS, pH 7.4, 0.09% Sodium Azide
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.