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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MICA/B Antibody (clone 6D4) LS‑C106571
B antibody LS-C106571 is an unconjugated mouse monoclonal antibody to human B (MICA). Validated for Flow and IP.
Catalog
Size
Price
LS-C106571-25
25 µg
$215
LS-C106571-100
100 µg
$245
Description
B antibody LS-C106571 is an unconjugated mouse monoclonal antibody to human B (MICA). Validated for Flow and IP.
Target
Human MICA/B
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [6D4]
Conjugations
Unconjugated. Also available conjugated with PE.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Immunoprecipitation
  • Flow Cytometry
  • (applications tested for the base form of this product only)
Failed Applications
  • IHC - Paraffin
Immunogen
MICA/B antibody was raised against human MICA
Usage
This 6D4 antibody has been tested by flow cytometric analysis of HeLa cells. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Presentation
PBS, pH 7.2, 150 mM NaCl, 0.09% Sodium Azide
Storage
Store at 4°C, avoid freeze-thaw cycles.
Restrictions
For research use only.

Popular MICA/B Products

Human Hela cell line stained with purified 6D4, followed by anti-mouse IgG FITC
Species: Human
Applications: IHC, Flow Cytometry
Antibody
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Antibody
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Staining of HeLa cells with PE Mouse IgG2a, K isotype control (open histogram) or PE anti-human MICA/MICB (6D4) (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry
Antibody
Species: Human
Applications: IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry, Functional Assay

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Images

Flow Cytometry

Staining of HeLa cells with 0.25 ug of Purified Mouse IgG2a, K isotype control (open histogram) or 0.25 ug of Purified anti-human MICA/MICB (6D4) (colored histogram) followed by FITC Anti-Mouse IgG. Total viable cells were used for analysis.
Staining of HeLa cells with 0.25 ug of Purified Mouse IgG2a, K isotype control (open histogram) or 0.25 ug of Purified anti-human MICA/MICB (6D4) (colored histogram) followed by FITC Anti-Mouse IgG. Total viable cells were used for analysis.

Flow Cytometry

Staining of HeLa cells with 0.25 ug of Purified Mouse IgG2a, K isotype control (open histogram) or 0.25 ug of Purified anti-human MICA/MICB (6D4) (colored histogram) followed by FITC Anti-Mouse IgG. Total viable cells were used for analysis.
Staining of HeLa cells with 0.25 ug of Purified Mouse IgG2a, K isotype control (open histogram) or 0.25 ug of Purified anti-human MICA/MICB (6D4) (colored histogram) followed by FITC Anti-Mouse IgG. Total viable cells were used for analysis.

Flow Cytometry

Staining of HeLa cells with 0.25 ug of Purified Mouse IgG2a, K isotype control (open histogram) or 0.25 ug of Purified anti-human MICA/MICB (6D4) (colored histogram) followed by FITC Anti-Mouse IgG. Total viable cells were used for analysis.
Staining of HeLa cells with 0.25 ug of Purified Mouse IgG2a, K isotype control (open histogram) or 0.25 ug of Purified anti-human MICA/MICB (6D4) (colored histogram) followed by FITC Anti-Mouse IgG. Total viable cells were used for analysis.

Flow Cytometry

Staining of HeLa cells with 0.25 ug of Purified Mouse IgG2a, K isotype control (open histogram) or 0.25 ug of Purified anti-human MICA/MICB (6D4) (colored histogram) followed by FITC Anti-Mouse IgG. Total viable cells were used for analysis.
Staining of HeLa cells with 0.25 ug of Purified Mouse IgG2a, K isotype control (open histogram) or 0.25 ug of Purified anti-human MICA/MICB (6D4) (colored histogram) followed by FITC Anti-Mouse IgG. Total viable cells were used for analysis.

Requested From: United States
Date Requested: 12/15/2018
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