Rat MHC Class II RT-1B
(tested or 100% immunogen sequence identity)
- Flow Cytometry (1:1 - 1:10)
Rat thymocyte membrane glycoproteins.
Recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Reacts with certain mouse strains of MHC haplotypes k and s. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. The clone does not react with the Rat BDIX strain. This product is routinely tested in flow cytometry on rat splenocytes.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, pH 7.4, 0.09% Sodium Azide, 1% BSA
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
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