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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MHC Class II IA/IE Antibody (clone M5/114.15.2) LS‑C106929

Staining of C57Bl/6 splenocytes with 0.06 ug of Purified Rat IgG2b isotype control (open histogram) or 0.06 ug of Purified M5/114.15.2 (colored histogram) followed by FITC Anti-Rat IgG. Total viable cells were used for analysis.

MHC Class II IA/IE Antibody (clone M5/114.15.2) LS‑C106929

Antibody:
Rat Monoclonal to Mouse MHC Class II IA/IE
Application:
IHC, IHC-P, IHC-Fr, WB, Flo
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$215 
LS-C106929-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rat Monoclonal to Mouse MHC Class II IA/IE
Application:
IHC, IHC-P, IHC-Fr, WB, Flo
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
IE antibody LS-C106929 is an unconjugated rat monoclonal antibody to mouse IE (MHC Class II IA). Validated for Flow, IHC and WB.
Target
Mouse MHC Class II IA/IE
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal
Clone
M5/114.15.2
Conjugations
Unconjugated. Also available conjugated with FITC, Biotin, PE, PE, Cy5, APC.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Western blot
  • Flow Cytometry
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Mouse MHC Class II IA/IE
Usage
The M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions and can be used at less than or equal to 0.125 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Presentation
PBS, pH 7.2, 150 mM NaCl, 0.09% Sodium Azide
Storage
Store at 4°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 7/24/2019
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