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| Catalog Number | Size | Price |
|---|---|---|
| LS-C204829-0.1 | 0.1 mg (1 mg/ml) | $295 |
Monoclonal Rat anti‑Mouse MHC Class II I‑A/I‑E Antibody (clone M5/114, Azide‑free, IHC, WB) LS‑C204829
Monoclonal Rat anti‑Mouse MHC Class II I‑A/I‑E Antibody (clone M5/114, Azide‑free, IHC, WB) LS‑C204829
Antibody:
MHC Class II I-A/I-E Rat anti-Mouse Monoclonal (Azide-free) (M5/114) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, IP, Flo, Func
Reactivity:
Mouse
Format:
Unconjugated, Azide-free
Other formats:
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
MHC Class II I-A/I-E Rat anti-Mouse Monoclonal (Azide-free) (M5/114) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, IP, Flo, Func
Reactivity:
Mouse
Format:
Unconjugated, Azide-free
Other formats:
Specifications
Description
I-E antibody LS-C204829 is an unconjugated rat monoclonal antibody to mouse I-E (MHC Class II I-A). Validated for Flow, Func, IHC, IP and WB.
Host
Rat
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Clone
M5/114
Conjugations
Unconjugated.
Also available conjugated with FITC.
Purification
Purified by protein-G affinity chromatography. Greater than 95% by SDS-PAGE.
Modifications
Azide-free
Immunogen
Activated C57BL/6 mouse spleen cells.
Specificity
The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
Applications
- IHC
- IHC - Paraffin
- IHC - Frozen
- Western blot
- Immunoprecipitation
- Flow Cytometry
- Functional Assay
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Presentation
Sterile PBS, pH 7.4, Azide free
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Species:
Human, Monkey, Bovine, Guinea pig, Hamster, Pig
Applications:
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Species:
Bovine, Human, Mouse, Rat
Applications:
Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
Date Requested: 4/23/2024
