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Catalog Number Size Price
LS-C775649-100 100 µg (1 mg/ml) $531 
MGO / Methylglyoxal Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9F11. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody  at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
MGO / Methylglyoxal Antibody - Western Blot analysis of Methylglyoxal-BSA Conjugate showing detection of 67 kDa Methylglyoxal protein using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9F11. Lane 1: Molecular Weight Ladder (MW). Lane 2: Methylglyoxal-BSA. Lane 3: BSA. Load: 0.5 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
MGO / Methylglyoxal Antibody - Flow Cytometry analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9F11. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody  at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.
MGO / Methylglyoxal Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9F11. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody  at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
MGO / Methylglyoxal Antibody - Western Blot analysis of Methylglyoxal-BSA Conjugate showing detection of 67 kDa Methylglyoxal protein using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9F11. Lane 1: Molecular Weight Ladder (MW). Lane 2: Methylglyoxal-BSA. Lane 3: BSA. Load: 0.5 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
MGO / Methylglyoxal Antibody - Flow Cytometry analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9F11. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody  at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.
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Monoclonal Mouse anti‑All species MGO / Methylglyoxal Antibody (clone 9F11, Biotin, IF, WB) LS‑C775649

Monoclonal Mouse anti‑All species MGO / Methylglyoxal Antibody (clone 9F11, Biotin, IF, WB) LS‑C775649

Antibody:
MGO / Methylglyoxal Mouse Monoclonal (Biotin) (9F11) Antibody
Application:
IF, WB, Flo, ELISA
Reactivity:
All species
Format:
Biotin, Unmodified
Other formats:
Price
Catalog Number
$531
LS-C775649-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MGO / Methylglyoxal Mouse Monoclonal (Biotin) (9F11) Antibody
Application:
IF, WB, Flo, ELISA
Reactivity:
All species
Format:
Biotin, Unmodified
Other formats:

Specifications

Description
Methylglyoxal antibody LS-C775649 is a biotin-conjugated mouse monoclonal antibody to all species Methylglyoxal (MGO). Validated for ELISA, Flow, IF and WB.
Target
All species MGO / Methylglyoxal
Host
Mouse
Reactivity
All species
Clonality
IgG1 Monoclonal
Clone
9F11
Conjugations
Biotin. Also available conjugated with AP, APC, Atto 390, Atto 488, Atto 594, FITC, PerCP, RPE.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH).
Specificity
Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdeyhde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crotonaldehyde modified proteins.
Applications
  • Immunofluorescence (1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:50)
  • ELISA (1:1000)
  • Applications tested for the base form of this product only
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 4/19/2024