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Catalog Number Size Price
LS-C662245-10 10 µg $318 
LS-C662245-100 100 µg $470 
MED4 Antibody - Immunohistochemistry - Anti-MED4 Picoband Antibody
MED4 Antibody - Immunohistochemistry - Anti-MED4 Picoband Antibody
MED4 Antibody - Immunohistochemistry - Anti-MED4 Picoband Antibody
MED4 Antibody - Immunohistochemistry - Anti-MED4 Picoband Antibody
MED4 Antibody - Immunohistochemistry - Anti-MED4 Picoband Antibody
MED4 Antibody - Immunohistochemistry - Anti-MED4 Picoband Antibody
MED4 Antibody - Western blot analysis of MED4 using anti-MED4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED4 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MED4 at approximately 34KD. The expected band size for MED4 is at 30KD.
MED4 Antibody - Western blot - Anti-MED4 Picoband Antibody
MED4 Antibody - Immunohistochemistry - Anti-MED4 Picoband Antibody
MED4 Antibody - Immunohistochemistry - Anti-MED4 Picoband Antibody
MED4 Antibody - Immunohistochemistry - Anti-MED4 Picoband Antibody
MED4 Antibody - Immunohistochemistry - Anti-MED4 Picoband Antibody
MED4 Antibody - Immunohistochemistry - Anti-MED4 Picoband Antibody
MED4 Antibody - Immunohistochemistry - Anti-MED4 Picoband Antibody
MED4 Antibody - Western blot analysis of MED4 using anti-MED4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED4 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MED4 at approximately 34KD. The expected band size for MED4 is at 30KD.
MED4 Antibody - Western blot - Anti-MED4 Picoband Antibody
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Polyclonal Rabbit anti‑Human MED4 Antibody (IHC, WB) LS‑C662245

Polyclonal Rabbit anti‑Human MED4 Antibody (IHC, WB) LS‑C662245

Antibody:
MED4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662245-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MED4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
MED4 antibody LS-C662245 is an unconjugated rabbit polyclonal antibody to human MED4. Validated for ELISA, IHC and WB.
Target
Human MED4
Synonyms
MED4 | ARC36 | DRIP36 | HSPC126 | Mediator complex subunit 4 | TRAP/SMCC/PC2 subunit p36 | TRAP36 | VDRIP
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human MED4 recombinant protein (Position: R27-K140).
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MED4
Q9NPJ6 NM_014166 NP_054885.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024