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Catalog Number Size Price
LS-C368414-30 30 µl (1 mg/ml) $255 
LS-C368414-100 100 µl (1 mg/ml) $299 
LS-C368414-200 200 µl (1 mg/ml) $351 
MED24 / TRAP100 Antibody - Immunohistochemical analysis of TRAP100 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
MED24 / TRAP100 Antibody - Immunofluorescent analysis of TRAP100 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MED24 / TRAP100 Antibody - Western blot analysis of TRAP100 expression in HepG2 (A); HEK293T (B); HeLa (C) whole cell lysates.
MED24 / TRAP100 Antibody - Immunohistochemical analysis of TRAP100 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
MED24 / TRAP100 Antibody - Immunofluorescent analysis of TRAP100 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MED24 / TRAP100 Antibody - Western blot analysis of TRAP100 expression in HepG2 (A); HEK293T (B); HeLa (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human MED24 / TRAP100 Antibody (C‑Terminus, IHC, IF, WB) LS‑C368414

Polyclonal Rabbit anti‑Human MED24 / TRAP100 Antibody (C‑Terminus, IHC, IF, WB) LS‑C368414

Antibody:
MED24 / TRAP100 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Rat, Bovine, Pig, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$255
LS-C368414-30
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MED24 / TRAP100 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Rat, Bovine, Pig, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
TRAP100 antibody LS-C368414 is an unconjugated rabbit polyclonal antibody to TRAP100 (MED24) (C-Terminus) from human. It is reactive with human, rat, bovine and other species. Validated for ICC, IF, IHC and WB.
Target
Human MED24 / TRAP100
Synonyms
MED24 | ARC100 | CRSP complex subunit 4 | CRSP4 | Mediator complex subunit 24 | HTRAP100 | KIAA0130 | THRAP4 | TRAP100 | CRSP100 | DRIP100
Host
Rabbit
Reactivity
Human, Rat, Bovine, Pig, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human TRAP100.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of TRAP100 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MED24 / TRAP100
O75448 NM_014815 NP_055630.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/16/2024