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Antibodies > MED1 / TRAP220 > LS-B14558
 
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Catalog Number Size Price
LS-B14558-50 50 µl (1 mg/ml) $460 
MED1 / TRAP220 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
MED1 / TRAP220 Antibody - Immunofluorescent analysis of TRAP220 staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MED1 / TRAP220 Antibody - Western blot analysis of TRAP220 expression in HeLa (A); HUVEC (B); Jurkat (C) whole cell lysates.
MED1 / TRAP220 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
MED1 / TRAP220 Antibody - Immunofluorescent analysis of TRAP220 staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MED1 / TRAP220 Antibody - Western blot analysis of TRAP220 expression in HeLa (A); HUVEC (B); Jurkat (C) whole cell lysates.
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IHC‑plus™ Polyclonal Rabbit anti‑Human MED1 / TRAP220 Antibody (Internal, IHC, IF, WB) LS‑B14558

IHC‑plus™ Polyclonal Rabbit anti‑Human MED1 / TRAP220 Antibody (Internal, IHC, IF, WB) LS‑B14558

IHC Validated
(4.0) Rating
Submit Review LSBio Guarantee
Note: This antibody replaces LS-C352686
Antibody:
MED1 / TRAP220 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B14558-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
MED1 / TRAP220 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
TRAP220 antibody LS-B14558 is an unconjugated rabbit polyclonal antibody to TRAP220 (MED1) (Internal) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human MED1 / TRAP220
Synonyms
MED1 | ARC205 | CRSP1 | DRIP205 | DRIP230 | Mediator complex subunit 1 | PBP | PPARG binding protein | RB18A | TRIP-2 | TRIP2 | PPARBP | CRSP200 | p53 regulatory protein RB18A | PPAR binding protein | PPAR-binding protein | PPARGBP | TR-interacting protein 2 | TRAP220
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human TRAP220.
Epitope
Internal
Specificity
Recognizes endogenous levels of TRAP220 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MED1 / TRAP220
Q15648 NM_004774 NP_004765.2

Validation

MED1 / TRAP220 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
About LSBio Validation process

LSBio Ratings

IHC-plus™ MED1 / TRAP220 Antibody (Internal) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-B14558 has an LSBio Rating of
(4.0) Rating
Laboratory Validation Score (4)

Publications (0)

Submit a Publication for LS-B14558

Customer Reviews (0)

Submit a review for LS-B14558

Featured Products

MED1 / TRAP220 Antibody - Detection of Human and Mouse CRSP1/TRAP220 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibodies: Affinity purified goat anti-CRSP1/TRAP220 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
MED1 / TRAP220 Antibody (aa1531‑1581) LS‑C290523
Species: Human, Mouse
Applications: IHC, Western blot, Immunoprecipitation
MED1 / TRAP220 Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MED1 Antibody. The picture on the right is blocked with the synthesized peptide.
MED1 / TRAP220 Antibody (aa641‑690) LS‑C119067
Species: Human, Mouse
Applications: IHC, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
MED1 / TRAP220 Antibody - Immunofluorescence analysis of HeLa cells, using PPAR-BP Antibody. The picture on the right is blocked with the synthesized peptide.
MED1 / TRAP220 Antibody (aa1423‑1472) LS‑C117699
Species: Human, Mouse
Applications: Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
MED1 / TRAP220 Antibody - Immunofluorescence of monoclonal antibody to PPARBP on HeLa cell . [antibody concentration 10 ug/ml]
MED1 / TRAP220 Antibody (clone 2A2) LS‑C197960
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
MED1 / TRAP220 Antibody - Immunohistochemical analysis of TRAP220 (pT1457) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
MED1 / TRAP220 Antibody (phospho‑Thr1457) LS‑C358800
Species: Human, Monkey, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
MED1 / TRAP220 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
Human MED1 / TRAP220 Protein (Recombinant GST, N-terminus) (aa1391-1490) - LS-G59680
Source: Wheat
Tag: GST, N-terminus
See all MED1 / TRAP220 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
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