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Catalog Number Size Price
LS-C745166-100 100 µg (1 mg/ml) $567 
ME1 / Malate Dehydrogenase Antibody - Western Blot of Sheep anti-Malate Dehydrogenase antibody. Lane 1: Malate Dehydrogenase. Lane 2: None. Load: 50 ng per lane. Primary antibody: Malate Dehydrogenase antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase sheep secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 36 kDa, 36 kDa for Malate Dehydrogenase. Other band(s): None.

Polyclonal Sheep anti‑Pig ME1 / Malate Dehydrogenase Antibody (Lyophilized, WB) LS‑C745166

Polyclonal Sheep anti‑Pig ME1 / Malate Dehydrogenase Antibody (Lyophilized, WB) LS‑C745166

Antibody:
ME1 / Malate Dehydrogenase Sheep anti-Pig Polyclonal (Lyophilized) Antibody
Application:
WB, IP, ELISA
Reactivity:
Pig
Format:
Unconjugated, Lyophilized
Price
Catalog Number
$567
LS-C745166-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ME1 / Malate Dehydrogenase Sheep anti-Pig Polyclonal (Lyophilized) Antibody
Application:
WB, IP, ELISA
Reactivity:
Pig
Format:
Unconjugated, Lyophilized

Specifications

Description
Malate Dehydrogenase antibody LS-C745166 is an unconjugated sheep polyclonal antibody to pig Malate Dehydrogenase (ME1). Validated for ELISA, IP and WB.
Target
Pig ME1 / Malate Dehydrogenase
Synonyms
ME1 | HUMNDME | Malate dehydrogenase | Malic enzyme 1 | Malate oxidoreductase | Malic enzyme 1, soluble | MES | NADP-ME | Pyruvic-malic carboxylase | NADP-dependent malic enzyme | Malic enzyme, cytoplasmic
Host
Sheep
Reactivity
Pig (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Malate Dehydrogenase [Pig Heart].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart]. Cross reactivity against Malate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
Applications
  • Western blot (1:500 - 1:5000)
  • Immunoprecipitation (1:100)
  • ELISA (1:5000 - 1:20000)
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ME1 / Malate Dehydrogenase
P48163 NM_002395 NP_002386.1

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Requested From: United States
Date Requested: 4/24/2024