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Catalog Number Size Price
LS-C19046-100 100 µg (1 mg/ml) $498 
MDM2 Antibody - Anti-MDM2 Antibody - Western Blot. Western blot using affinity purified Anti-MDM2 (Rabbit) is shown to detect a band (arrow) corresponding to mouse MDM2 protein present in mouse MEF cells (lane 2) but not human kidney HEK293 cells (lane 1). Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking the membrane with 5% normal goat serum, 0.5% BLOTTO in PBS, the membrane was probed for overnight at 4° with the primary antibody diluted to 1:500 in 1% normal goat serum, 0.1% BLOTTO in PBS. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (700 nm channel, red). IRDye800 fluorescence image was captured using the Ddyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Polyclonal Rabbit anti‑Mouse MDM2 Antibody (IHC, WB) LS‑C19046

Polyclonal Rabbit anti‑Mouse MDM2 Antibody (IHC, WB) LS‑C19046

Antibody:
MDM2 Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$498
LS-C19046-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MDM2 Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
MDM2 antibody LS-C19046 is an unconjugated rabbit polyclonal antibody to mouse MDM2. Validated for ELISA, IHC, IP and WB.
Target
Mouse MDM2
Synonyms
MDM2 | ACTFS | Double minute 2 protein | Hdm2 | MDM2 variant FB30 | HDMX | Oncoprotein Mdm2 | p53-binding protein Mdm2 | MDM2 variant FB28
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 175-200 of mouse MDM2.
Specificity
A BLAST analysis was used to suggest minimal cross reactivity with MDM2 homologues from other sources.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:100)
  • ELISA (1:3000 - 1:12000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 90 kD in size corresponding to MDM2 protein by western blotting in the appropriate cell lysate or extract. See Saucedo, et al (1999) for a discussion on expected molecular weights.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MDM2
Q00987 NM_002392 NP_002383.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/13/2022