MDM2 | ACTFS | Double minute 2 protein | Hdm2 | MDM2 variant FB30 | HDMX | Oncoprotein Mdm2 | p53-binding protein Mdm2 | MDM2 variant FB28
(tested or 100% immunogen sequence identity)
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 175-200 of mouse MDM2.
A BLAST analysis was used to suggest minimal cross reactivity with MDM2 homologues from other sources.
- IHC - Paraffin (5 µg/ml)
- Western blot (1:500 - 1:2000)
- Immunoprecipitation (1:100)
- ELISA (1:3000 - 1:12000)
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This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 90 kD in size corresponding to MDM2 protein by western blotting in the appropriate cell lysate or extract. See Saucedo, et al (1999) for a discussion on expected molecular weights.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.