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MDK / Midkine

midkine (neurite growth-promoting factor 2)

Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development.

Gene Name: midkine (neurite growth-promoting factor 2)
Synonyms: MDK, ARAP, MK, MK1, NEGF2, Midkine, Retinoic acid inducible factor
Target Sequences: NM_002391 NP_002382.1 P21741

Publications (3)

1
High-level soluble expression, purification and characterization of active human midkine from Escherichia coli. Yan WK, Goette M, Hofmann G, Zaror I, Sim J. Protein expression and purification. 2010 70:270-6. (WB) [PubMed:19883768]
2
DNA damage repair alterations modulate M2 polarization of microglia to remodel the tumor microenvironment via the p53-mediated MDK expression in glioma. Meng X, Duan C, Pang H, Chen Q, Han B, Zha C, Dinislam M, Wu P, Li Z, Zhao S, Wang R, Lin L, Jiang C, Cai J. EBioMedicine. 2019 March;41:185-199. [Full Text Article] [PubMed:30773478] [PMC:PMC6442002]
3
Intratumoural heterogeneity generated by Notch signalling promotes small-cell lung cancer. Lim JS, Ibaseta A, Fischer MM, Cancilla B, O'Young G, Cristea S, Luca VC, Yang D, Jahchan NS, Hamard C, Antoine M, Wislez M, Kong C, Cain J, Liu YW, Kapoun AM, Garcia KC, Hoey T, Murriel CL, Sage J. Nature. 2017 May;545:360-364. [Full Text Article] [PubMed:28489825] [PMC:PMC5776014]

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MDK / Midkine Antibody - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
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MDK / Midkine Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
50 µl/$460
MDK / Midkine Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MDK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MDK (1:2000).
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MDK / Midkine Mouse anti-Human Monoclonal (aa21-143) (OTI6C8) Antibody
Human
WB
Unconjugated
100 µl/$434
MDK / Midkine Antibody - MDK Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human pancreas tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
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MDK / Midkine Rabbit anti-Human Polyclonal (aa113-143) Antibody
Mouse, Human
IF, IHC, IHC-P, WB
Unconjugated
400 µl/$393
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MDK / Midkine Mouse anti-Human Monoclonal (Preservative Free) (3G1-E1-G10-G8-D9-F3) Antibody
Human
ELISA, WB
Unconjugated, Preservative Free
20 µg/$242; 100 µg/$304; 200 µg/$334; 500 µg/$452
MDK / Midkine ELISA Kit
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Sandwich
96-Well Strip Plate
Mouse
78 - 5000 pg/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$783
MDK / Midkine ELISA Kit
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Sandwich
96-Well Strip Plate
Mouse
0.156 - 10 ng/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates
1 Plate/$782
MDK / Midkine ELISA Kit
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Sandwich
12 x 8-Well Microstrips
Human
32 - 2000 pg/ml
Colorimetric - 450nm (TMB)
Cell Lysates, Plasma, Serum
1 Plate/$479
MDK / Midkine Antibody - Western blot of recombinant MDK / Midkine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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MDK / Midkine Rabbit anti-Mouse Polyclonal (aa22-140) Antibody
Mouse
WB
Unconjugated
100 µl/$301; 200 µl/$340; 500 µl/$470; 1 ml/$535; 2 ml/$698; 5 ml/$1,161
MDK / Midkine Antibody - Western blot of recombinant MDK / Midkine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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MDK / Midkine Rabbit anti-Mouse Polyclonal (aa22-140) (PE) Antibody
Mouse
WB
PE Conjugated
100 µl/$392; 200 µl/$470; 500 µl/$798; 1 ml/$951; 2 ml/$1,211; 5 ml/$2,269
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MDK / Midkine Mouse anti-Human Monoclonal (Preservative Free) Antibody
Human
ELISA, WB
Unconjugated, Preservative Free
20 µg/$242; 100 µg/$304; 200 µg/$334; 500 µg/$452
MDK / Midkine Antibody - Anti-Midkine antibody IHC of human small intestine, goblet cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
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MDK / Midkine Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µg/$485
MDK / Midkine ELISA Kit
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Sandwich
96-Well Microplate
Human
0.123 - 30 ng/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Plasma, Serum
1 Plate/$700
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MDK / Midkine Mouse anti-Human Monoclonal (Preservative Free) (07) Antibody
Human
ELISA
Unconjugated, Preservative Free
100 µl/$412
MDK / Midkine Antibody - Western blot of recombinant MDK / Midkine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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MDK / Midkine Mouse anti-Human Monoclonal (aa22-143) (C1) Antibody
Human
WB
Unconjugated
100 µl/$305; 200 µl/$345; 500 µl/$479; 1 ml/$547; 2 ml/$725; 5 ml/$1,192
MDK / Midkine Protein
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E. coli
None
Biologically active
Less than 1.0 EU/µg protein (determined by LAL method).
~14 kDa
20 µg/$301; 100 µg/$490; 1 mg/$1,891
MDK / Midkine Antibody - 1:100 staining human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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MDK / Midkine Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
MDK / Midkine ELISA Kit
Select
Development Kit
1000 Wells
Human
32 - 2000 pg/ml
Colorimetric - 405nm (ABTS)
1 Test/$540
MDK / Midkine ELISA Kit
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Competitive EIA
96-Well Strip Plate
Human
0.5 - 10 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$913
MDK / Midkine ELISA Kit
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Sandwich
96-Well Strip Plate
Rat
0.156 - 10 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$818
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MDK / Midkine Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC
Unconjugated
100 µg/$331
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MDK / Midkine Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
50 µg/$657
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MDK / Midkine Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
0.05 mg/$386
MDK / Midkine Antibody - Immunohistochemistry of paraffin-embedded Human lung cancer using MDK Polyclonal Antibody at dilution of 1:60.
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MDK / Midkine Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
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MDK / Midkine Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Human
ELISA, WB
Unconjugated, Preservative Free
20 µg/$242; 100 µg/$304; 200 µg/$334; 500 µg/$452
MDK / Midkine Antibody - Western blot analysis of lysate from SW480 cells, using MDK Antibody.
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MDK / Midkine Rabbit anti-Human Polyclonal Antibody
Rat, Human
ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
Viewing 1-25 of 139 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).