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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C14265-100 100 µg $755 
Antibody

Monoclonal Mouse anti‑Human MCAM / CD146 Antibody (PE, IHC) LS‑C14265

Monoclonal Mouse anti‑Human MCAM / CD146 Antibody (PE, IHC) LS‑C14265

Antibody:
Mouse Monoclonal to Human MCAM / CD146
Application:
IHC, IHC-Fr, ICC, Flo
Reactivity:
Human, Mouse, Dog, Rabbit
Format:
Phycoerythrin, Unmodified
Price
Catalog Number
$755 
LS-C14265-100
100 µg
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human MCAM / CD146
Application:
IHC, IHC-Fr, ICC, Flo
Reactivity:
Human, Mouse, Dog, Rabbit
Format:
Phycoerythrin, Unmodified

Specifications

Description
CD146 antibody LS-C14265 is a PE-conjugated mouse monoclonal antibody to CD146 (MCAM) from human. It is reactive with human, mouse, dog and other species. Validated for Flow, ICC and IHC.
Target
Human MCAM / CD146
Synonyms
MCAM | CD146 | CD146 antigen | Gicerin | Melanoma adhesion molecule | MUC18 | p1h12
Host
Mouse
Reactivity
Human, Mouse, Dog, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Phycoerythrin
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • ICC (1 - 10 µg/ml)
  • Flow Cytometry (1 - 10 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HUVEC.
Specificity
Recognizes human CD146 {MCAM, MUC18}. In blood and marrow, reacts only with endothelial cells. Species cross-reactivity: canine, rabbit and mouse. Does not cross-react with rat.
Usage
Suitable for use in FACS, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Flow Cytometry: 1-10 ug/ml. Immunocytochemistry: 1-10 ug/ml, EDTA- or trypsin-lifted endothelial cells. Immunohistochemistry (Frozen, fixed or unfixed): 1-10 ug/ml. For fixative, use 4% PFA for 30 minutes RT or 4?C. for 2hrs. Block with 1% BSA, 0.2% Tween-20, PBS for 30 minutes. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.6, 15 mg/ml BSA, 0.1% sodium azide.
Storage
Do not freeze. Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing PE conjugates will result in a substantial loss of enzymatic activity.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MCAM / CD146
P43121 NM_006500 NP_006491.2

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Requested From: United States
Date Requested: 7/15/2020