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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C14268-100 100 µg $635 
Antibody

Monoclonal Mouse anti‑Human MCAM / CD146 Antibody (IHC, WB) LS‑C14268

Monoclonal Mouse anti‑Human MCAM / CD146 Antibody (IHC, WB) LS‑C14268

Antibody:
Mouse Monoclonal to Human MCAM / CD146
Application:
IHC, IHC-Fr, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$635 
LS-C14268-100
100 µg
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human MCAM / CD146
Application:
IHC, IHC-Fr, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
CD146 antibody LS-C14268 is an unconjugated mouse monoclonal antibody to human CD146 (MCAM). Validated for ELISA, Flow, IHC and WB.
Target
Human MCAM / CD146
Synonyms
MCAM | CD146 | CD146 antigen | Gicerin | Melanoma adhesion molecule | MUC18 | p1h12
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • Western blot (1 - 10 µg/ml)
  • Flow Cytometry (5 - 10 µg/ml)
  • ELISA (0.1 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant human MUC18 (D1-D5) Fc protein.
Specificity
Reacts with the human MUC18 (CD146, Mel-CAM, MCAM) protein. Reactivity is confirmed with human SK-Mel-37 melanoma cell lysates.
Usage
Suitable for use in Immunohistochemistry, Flow Cytometry, ELISA and Western Blot. Immunohistochemistry (frozen): 5-10 ug/ml. Flow Cytometry: 5-10 ug/ml. ELISA: 0.1-1 ug/ml. Western Blot: 1-10 ug/ml.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MCAM / CD146
P43121 NM_006500 NP_006491.2

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Requested From: United States
Date Requested: 7/6/2020