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| Catalog Number | Size | Price |
|---|---|---|
| LS-B4050-50 | 50 µg (0.5 mg/ml) | $460 |
1 of 2
2 of 2
IHC‑plus™ Monoclonal Mouse anti‑Human MCAM / CD146 Antibody (clone P1H12, IHC, WB) LS‑B4050
IHC‑plus™ Monoclonal Mouse anti‑Human MCAM / CD146 Antibody (clone P1H12, IHC, WB) LS‑B4050
Note: This antibody replaces LS-C106635, LS-C14262
Antibody:
MCAM / CD146 Mouse anti-Human Monoclonal (P1H12) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, IP, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
MCAM / CD146 Mouse anti-Human Monoclonal (P1H12) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, IP, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
CD146 antibody LS-B4050 is an unconjugated mouse monoclonal antibody to human CD146 (MCAM). Validated for Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human MCAM / CD146
Synonyms
MCAM | CD146 | CD146 antigen | Gicerin | Melanoma adhesion molecule | MUC18 | p1h12
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
P1H12
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Human umbilical vein cells
Applications
- IHC
- IHC - Paraffin (20 µg/ml)
- IHC - Frozen (1 - 10 µg/ml)
- Western blot (1 - 5 µg/ml)
- Immunoprecipitation
- Flow Cytometry
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
This P1H12 antibody has been pre-titrated and tested by flow cytometric analysis of HUVEC cell line. This can be used at 0.25 ul per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Presentation
Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.
Storage
Store at 4°C, avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About MCAM / CD146
Validation
Anti-MCAM / CD146 antibody IHC of human vessels in human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Anti-MCAM / CD146 antibody IHC of human vessels in human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ MCAM / CD146 Antibody (clone P1H12) for IHC, WB/Western, IP, Flow LS-B4050 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Melanoma Cell Galectin-1 Ligands Functionally Correlate with Malignant Potential. Yazawa EM, Geddes-Sweeney JE, Cedeno-Laurent F, Walley KC, Barthel SR, Opperman MJ, Liang J, Lin JY, Schatton T, Laga AC, Mihm MC, Qureshi AA, Widlund HR, Murphy GF, Dimitroff CJ. The Journal of investigative dermatology. 2015 135:1849-1862. (WB; Human)
Customer Reviews (0)
Featured Products
Species:
Rat
Applications:
Western blot, Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, Western blot, ELISA
Species:
Mouse
Applications:
Western blot, ELISA
Species:
Human, Mouse, Dog, Rabbit
Applications:
IHC, ICC, Immunoprecipitation, Flow Cytometry, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
Date Requested: 4/24/2024
