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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C152895-100 100 µg (1 mg/ml) $515 
Antibody

Monoclonal Mouse anti‑Human MCAM / CD146 Antibody (Biotin, IHC) LS‑C152895

Monoclonal Mouse anti‑Human MCAM / CD146 Antibody (Biotin, IHC) LS‑C152895

Antibody:
Mouse Monoclonal to Human MCAM / CD146
Application:
IHC, IHC-Fr, ICC, IP, Flo, ELISA
Reactivity:
Human, Mouse, Dog, Rabbit
Format:
Biotin, Unmodified
Price
Catalog Number
$515 
LS-C152895-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human MCAM / CD146
Application:
IHC, IHC-Fr, ICC, IP, Flo, ELISA
Reactivity:
Human, Mouse, Dog, Rabbit
Format:
Biotin, Unmodified

Specifications

Description
CD146 antibody LS-C152895 is a biotin-conjugated mouse monoclonal antibody to CD146 (MCAM) from human. It is reactive with human, mouse, dog and other species. Validated for ELISA, Flow, ICC, IHC and IP.
Target
Human MCAM / CD146
Synonyms
MCAM | CD146 | CD146 antigen | Gicerin | Melanoma adhesion molecule | MUC18 | p1h12
Host
Mouse
Reactivity
Human, Mouse, Dog, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Biotin
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • ICC
  • Immunoprecipitation
  • Flow Cytometry
  • ELISA (1 - 10 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Immunization of mice with HUVEC
Specificity
P1H12 reacts specifically with CD146 {MCAM, MUC18}. In blood and marrow P1H12 reacts only with endothelial cells, and has been used to detect circulating endothelial cells in human, dogs, rabbits and mouse samples. It positively stains normal, primary HUVEC and MVEC cultures and the endothelial cells of all vessels in normal frozen sections of human skin, intestine, ovary tonsil, lymph node, lung, and kidney. It does not stain carcinoma cell lines HT-29 and COLO205, and M21, the T cell lines Jurkat and HuT78, fibroblasts, HL-60 or CHO cells, or EBV-transformed B cell lines, although it expected that CD146 expression is present in many tumor lines. P1H12 does not stain normal monocytes, granulocytes, red cells, platelets, T cells or B cells from marrow or peripheral blood; nor does it detect marrow megakaryocytes or the megakaryoblast line HU3. The peripheral blood cells that do stain with P1H12 also are positive for both von Willebrand Factor (vWF) and thrombomodulin (the combined expression of which is limited to endothelium), and they stain for flt and flk. In recent testing, melanoma A2058, SKMEL.3 and A375. S2 are positive, while murine melanoma M3 is negative. One smooth muscle line (HISM) was positive and one negative (TIGHA-VSMC). Human blood lymphocytes are negative with or without prior stimulation. This biotinylated format is particularly useful for studies on mouse tissues when combined with streptavidin-FITC {SA103}
Usage
Immunocytochemistry: 1-10 ug/ml. Works best on EDTA or Trypsin lifted endothelial cells. Immunohistochemistry: 1-10 ug/ml. 4% PFA for 30 min RT or <2hrs @ 4°C. Block w/ 1%BSA/0.2%tween20/PBC for 30min. Works well in frozen tissue; fixed or unfixed. Immuno The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.02 M PBS, 0.25 M sodium chloride, pH=7.6, 15 mg/nL BSA, 0.1% sodium azide
Storage
Store at 4°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MCAM / CD146
P43121 NM_006500 NP_006491.2

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Requested From: United States
Date Requested: 6/5/2020