Human MBP / Myelin Basic Protein

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Ammonium sulfate precipitation

Unmodified

Human myelin basic protein from brain

Suitable for labeling of oligodendrocytes and myelin of white matter in brain and spinal cord (gray matter stains less abundantly). Also stains peripheral nerves.

• IHC
• IHC - Paraffin (1:100)
• Western blot (1:1500)

Immunohistochemistry: LS-B3112 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3112 was determined to be 1:100. Western Blot: 1:1500 in TTBS, overnight at 4uC.

0.05M Tris HCL, pH 7.6, 15 mM sodium azide before the addition of glycerol to 40%

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02686 NCBI: NM_002385 NP_002376.1