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Catalog Number Size Price
LS-C18889-100 100 µg (1 mg/ml) $498 
MARK2 Antibody - Western Blot of Rabbit anti-MARK2pT595 antibody. Lane 1: wild type Jurkat cells. Lane 2: wild type Jurkat cells stimulated with CD3/CD28 (T-cell receptor stimulation). Load: 35 µg per lane. Primary antibody: MARK2 pT595 antibody at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.

Polyclonal Rabbit anti‑Human MARK2 Antibody (phospho‑Thr595, IHC, IF, WB) LS‑C18889

Polyclonal Rabbit anti‑Human MARK2 Antibody (phospho‑Thr595, IHC, IF, WB) LS‑C18889

Antibody:
MARK2 Rabbit anti-Human Polyclonal (pThr595) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$498
LS-C18889-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MARK2 Rabbit anti-Human Polyclonal (pThr595) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
MARK2 antibody LS-C18889 is an unconjugated rabbit polyclonal antibody to MARK2 (pThr595) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB.
Target
Human MARK2
Synonyms
MARK2 | ELKL motif kinase 1 | EMK-1 | ELKL motif kinase | EMK1 | PAR-1 | PAR1 homolog | Par1b | Par-1b | Ser/Thr protein kinase PAR-1B | Emk | PAR1 homolog b
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bovine, Dog (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding T595 of Human MARK2 isoform a.  The immunogen peptide was phosphorylated at the residue corresponding to T595.
Epitope
pThr595
Specificity
Reactivity occurs against human MARK2 pT595 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human MARK2 is minimal by ELISA. Expect at least partial reactivity with other isoforms of MARK. The immunogen sequence is present in all forms identified to date and localizes to T595 on MARK2, T587 on MARK3, T591 on MARK1 and T568 on MARK4. A BLAST analysis was used to suggest reactivity with this protein from human, dog, bovine, mouse and rat sources based on 100% homology for the immunogen sequence. Cross reactivity with MARK2 homologues from other sources has not been determined.
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot (1:250 - 1:1000)
  • ELISA (1:1000 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 83 kD corresponding to MARK2 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MARK2
Q7KZI7 NM_017490 NP_059672.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/8/2022