Human MAPKAPK5 / PRAK

Goat

Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence C-STEALDNVLPSAQ, from the internal region of the protein sequence according to NP_003659.2NP_620777.1.

aa306-318

Human MAPKAPK5 / PRAK

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (0.5 - 1 µg/ml)
• Immunoprecipitation (1.5 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:32000)

Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP; only the expected band was observed in the MK5 lane after EB11927 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1 ug/ml. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with Mk5/Erk3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 ug EB11927 or EB11928. Western blots of the IP were labeled with mouse anti-MK5 (SC) or with rabbit anti-ERK3 serum (CST). An approx 55kD of MK5 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q8IW41 NCBI: NM_003668 NP_003659.2