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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C18843-100 100 µg (0.9 mg/ml) $425 
MAPKAPK2 / MAPKAP Kinase 2 Antibody - Anti-MAPKAP Kinase 2 Polyclonal Antibody - Western Blot. Affinity purified anti-MAPKAP Kinase 2 polyclonal antibody detects MK2 in unstimulated human HeLa whole cell lysate by western blot. Polyclonal rabbit-anti-MAPKAP Kinase 2 used at a 1:2000 dilution to detect 20 ug of whole cell lysate containing MK2. This antibody detects a single 44 kD protein as indicated in crude extracts prepared from either unstimulated or TNFa stimulated human HeLa cell lysates. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Polyclonal MAPKAPK2 / MAPKAP Kinase 2 Antibody (IHC, WB) LS‑C18843

Polyclonal MAPKAPK2 / MAPKAP Kinase 2 Antibody (IHC, WB) LS‑C18843

Note: This antibody replaces LS-C25409
Antibody:
Rabbit Polyclonal to Human MAPKAPK2 / MAPKAP Kinase 2
Application:
IHC, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C18843-100
100 µg
0.9 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human MAPKAPK2 / MAPKAP Kinase 2
Application:
IHC, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
MAPKAP Kinase 2 antibody LS-C18843 is an unconjugated rabbit polyclonal antibody to MAPKAP Kinase 2 (MAPKAPK2 ) from human, mouse and rat. Validated for ELISA, IHC, IP and WB.
Target
Human MAPKAPK2 / MAPKAP Kinase 2
Synonyms
MAPKAPK2 | MK-2 | MAP KAP Kinase 2 | MAPKAP kinase 2 | MK2 | MAPKAP-K2 | MAPKAPK-2
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • Western blot (1:2000)
  • Immunoprecipitation
  • ELISA (1:15000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of rabbit MAPKAP Kinase 2 conjugated to KLH using maleimide. A terminal cysteine residue was added to facilitate coupling.
Specificity
Cross reactivity with MAPKAP Kinase 2 from other sources has not been tested.
Usage
This antibody is suitable for western blotting and ELISA.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Dilute only prior to immediate use.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAPKAPK2 / MAPKAP Kinase 2
P49137 NM_004759 NP_004750.1

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Requested From: United States
Date Requested: 2/21/2020