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MAPKAPK2 / MAPKAP Kinase 2 Antibody (aa310‑325) LS‑C18843

Anti-MAPKAP Kinase 2 Polyclonal Antibody - Western Blot. Affinity purified anti-MAPKAP Kinase 2 polyclonal antibody detects MK2 in unstimulated human HeLa whole cell lysate by western blot. Polyclonal rabbit-anti-MAPKAP Kinase 2 used at a 1:2000 dilution to detect 20 ug of whole cell lysate containing MK2. This antibody detects a single 44 kD protein as indicated in crude extracts prepared from either unstimulated or TNFa stimulated human HeLa cell lysates. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

MAPKAPK2 / MAPKAP Kinase 2 Antibody (aa310‑325) LS‑C18843

Note: This antibody replaces LS-C25409
Antibody:
Rabbit Polyclonal to Rabbit MAPKAPK2 / MAPKAP Kinase 2
Application:
WB, ELISA
Reactivity:
Rabbit, Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C18843-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Rabbit MAPKAPK2 / MAPKAP Kinase 2
Application:
WB, ELISA
Reactivity:
Rabbit, Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
MAPKAP Kinase 2 antibody LS-C18843 is an unconjugated rabbit polyclonal antibody to MAPKAP Kinase 2 (MAPKAPK2) from human, mouse, rat and other species. Validated for ELISA and WB.
Target
Rabbit MAPKAPK2 / MAPKAP Kinase 2
Host
Rabbit
Reactivity
Rabbit, Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
Synthetic peptide from rabbit MAPKAPK2 / MK2.
Epitope
aa310-325
Specificity
Aa 310-325 of rabbit MAPKAP Kinase 2 conjugated to KLH using maleimide. A terminal cysteine residue was added to facilitate coupling.
Usage
This antibody is suitable for western blotting and ELISA.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Dilute only prior to immediate use.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAPKAPK2 / MAPKAP Kinase 2
P49137 NM_004759 NP_004750.1

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Featured Products

Reactivity: Human
Range: 0.156-10 ng/ml
Flow cytometry of T Cell Receptor Cbeta 1 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry, Functional Assay
Flow Cytometry Image
Species: Mouse
Applications: Immunoprecipitation, Flow Cytometry

Requested From: United States
Date Requested: 7/20/2019
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